Anti-Firefly Luciferase抗体[3i1] (ab64564)

概述

  • 产品名称Anti-Firefly Luciferase抗体[3i1]
    参阅全部 Firefly Luciferase 一抗
  • 描述
    小鼠单克隆抗体[3i1] to Firefly Luciferase
  • 经测试应用适用于: WB, ELISA, ICCmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Firefly (Photinus pyralis)
  • 免疫原

    Full-length recombinant luciferase from Photinus pyralis.

  • 阳性对照
    • 3T3 cell lysate containing 5ng of recombinant luciferase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 40% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 0.1M Tris glycine, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号3i1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC
  • 应用说明ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    Is unsuitable for IHC-P.
    WB: Use at a concentration of 0.2 - 5 µg/ml. Predicted molecular weight: 66 kDa.
    Note: 0.5-2 µg/ml detects 5ng of recombinant luciferase that was added to 3T3 cell lysate.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
    • 细胞定位Peroxisome
    • 数据库链接
      • 别名
        • ec 1.13.12.7 antibody
        • Firefly antibody
        • Luciferase antibody
        • Luciferin 4 monooxygenase antibody

      Anti-Firefly Luciferase antibody [3i1] (ab64564)参考文献

      ab64564 has not yet been referenced specifically in any publications.

      Product Wall

      Application Western blot
      Loading amount 0.05 µg
      Gel Running Conditions Reduced Denaturing (4-15% Tris-glycine)
      Sample Firefly (Photinus pyralis) Recombinant protein (QuantiLum Recombinant Luciferase (Promega))
      Specification QuantiLum Recombinant Luciferase (Promega)
      Treatment Heated in the presence of sHSP followed by UV crosslinking
      Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Username

      Miss. Rachel Loh

      Verified customer

      提交于 Jan 13 2015

      We have no firefly luciferase antibody tested in IP which is mouse or rabbit origin. Only ab498- Goat polyclonal to firefly Luciferase Other Firefly Luciferase antibodies tested in WB: 1. ab16466 Firefly Luciferase antibody [Luci17] Mouse monocl...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"