Anti-FIP1L1抗体(ab80272)

概述

  • 产品名称Anti-FIP1L1抗体
    参阅全部 FIP1L1 一抗
  • 描述
    兔多克隆抗体to FIP1L1
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 544 and 594 of Human FIP1L1 (NP_112179.2).

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab80272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性FIP1L1 is an integral subunit of the cleavage and polyadenylation specificity factor (CPSF) complex. CPSF is a multisubunit complex that binds to the canonical AAUAAA hexamer and to U-rich upstream sequence elements on the pre-mRNA and interacts with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 binds to U-rich RNA sequence elements surrounding the poly(A) site and contributes to poly(A) site recognition and stimulates poly(A) addition. FIP1L1 may act to tether poly(A) polymerase to the CPSF complex. A fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA) is a recurrent molecular lesion in eosinophilia-associated myeloproliferative disorders.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • DKFZp586K0717 antibody
    • Factor interacting with PAP antibody
    • FIP1 antibody
    • FIP1 like 1 (S cerevisiae) antibody
    • FIP1 like 1 antibody
    • FLJ33619 antibody
    • hFip 1 antibody
    • hFip1 antibody
    • Pre mRNA 3 end processing factor FIP1 antibody
    • Rearranged in hypereosinophilia antibody
    • RHE antibody
    see all

Anti-FIP1L1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FIP1L1 with ab80272 at 1/5000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-FIP1L1 antibody (ab80272) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
    Additional bands at : 27 kDa,38 kDa,57 kDa. We are unsure as to the identity of these extra bands.
  • Detection of FIP1L1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80272 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-FIP1L1 antibody (ab80272)参考文献

ab80272 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Cell lysate - nuclear (human embryonic stem cells)
Specification human embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Miguel Fidalgo

Verified customer

提交于 Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"