Anti-Filamin C (phospho S2113)抗体(ab20485)

概述

  • 产品名称Anti-Filamin C (phospho S2113)抗体
    参阅全部 Filamin C 一抗
  • 描述
    兔多克隆抗体to Filamin C (phospho S2113)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Filamin C. The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Filamin C that contains serine 2146.

  • 阳性对照
    • C2C12, HeLa, HEK293 + EGF.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free) as a carrier. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Filamin C. The final product is generated by affinity chromatography using a Filamin C-derived peptide that is phosphorylated at serine 2113.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 291 kDa.

靶标

  • 相关性FLNC is a muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z-disks in muscle cells. Defects in FLNC are the cause of autosomal dominant filaminopathy. Myofibrillar myopathy (MFM) is a neuromuscular disorder, usually with an adult onset, characterized by focal myofibrillar destruction and pathological cytoplasmic protein aggregations. Autosomal dominant filaminopathy is a form of MFM characterized by morphological features of MFM and clinical features of a limb-girdle myopathy. A heterozygous nonsense mutation which segregates with the disease, has been identified in the FLNC gene.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • ABPL antibody
    • Actin binding like protein antibody
    • FIlamin 2 antibody
    • Filamin C antibody
    • FLN2 antibody
    • Gamma filamin antibody
    see all

Anti-Filamin C (phospho S2113) antibody 图像

  • All lanes : Anti-Filamin C (phospho S2113) antibody (ab20485) at 1/1000 dilution

    Lane 1 : HeLa cell lysate + Lambda phosphatase
    Lane 2 : HeLa cell lysate
    Lane 3 : HeLa cell lysate with a non-phosphorylated peptide corresponding to the immunogen
    Lane 4 : HeLa cell lysate with a generic phosphoserine-containing peptide
    Lane 5 : HeLa cell lysate with a phosphopeptide corresponding to Filamin C (phospho S2113)
    Lane 6 : Mouse myoblast cell lysate


    Predicted band size : 291 kDa

    Right panel: Lysates from HEK293 cells untreated (left lanes) or treated with EGF (right lanes). Western blotting was performed using the Filamin C (phospho S2113) antibody (upper blot) or an antibody recognizing total filamin protein (lower blot).

Anti-Filamin C (phospho S2113) antibody (ab20485)参考文献

ab20485 has not yet been referenced specifically in any publications.

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