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Our Abpromise guarantee covers the use of ab97457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 278 kDa.|
|ICC/IF||1/100 - 1/200.|
ab97547, at a 1/200 dilution, staining Filamin B in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
Immunofluorescence analysis of Human trabecular meshwork cells, staining Filamin B with ab97457.
Left panel: Filamin B staining alone. Right panel: Filamin B staining (red) merged with F-actin staining (green).
Cells were treated with dexamethasone, before fixing in paraformaldehyde and permeabilizing wih 0.2% Triton X-100. Cells were incubated with primary antibody (10 µg/ml) and AlexaFluor®488-conjugated phalloidin. Filamin B staining was detected using an AlexaFluor®546-conjugated goat anti-rabbit IgG.