概述

  • 产品名称Anti-Filamin抗体[FLMN01]
  • 描述
    小鼠单克隆抗体[FLMN01] to Filamin
  • 经测试应用适用于: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Guinea pig, Human
  • 免疫原

    Tissue, cells or virus corresponding to Filamin. Tissue/ cell preparation - platelet filamin

  • 表位not determined
  • 阳性对照
    • WB: NIH3T3 cells or mouse cells-2 lysate. IHC: Skin IF/ICC: A431 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab80837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 281 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-Filamin antibody [FLMN01] 图像

  • ICC/IF image of ab80837 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80837, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Filamin antibody [FLMN01] (ab80837) at 2 µg/ml + Mouse cells-2 lysate

    Predicted band size : 281 kDa
    This image is produced using the version of this antibody, containing BSA and azide.
  • Formalin-fixed, paraffin-embedded human skin stained with ab80837, at 1 - 2 µg/ml, using a peroxidase-conjugated secondary antibody and AEC chromogen. Note cytoplasmic staining of smooth muscle cells surrounding blood vessels.

    This image was produced using the version of this antibody, containing BSA and azide.
  • Overlay histogram showing A431 cells stained with ab80837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80837, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Filamin antibody [FLMN01] (ab80837)参考文献

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Thank you for your recent telephone enquiry and for your patience. It has taken some time to obtain the immunogen information for you.

For the two rabbit monoclonal antibodies:

ab76289
Immunogen: from within amino acids range...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"