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Full length native protein (purified) (Chicken).
Filamin is a large flexible actin cross-linking protein present in vertebrates in both muscle and non-muscle cells. Filamin has a polypeptide molecular weight of approximately 250,000 daltons and forms dimers in solution. Filamin has been primarily purified from smooth muscle but was also identified in macrophages, neutrophils, platelets, and other cell types. Filamin cross-links actin and promotes its aggregation and gelation. Immunofluorescent labeling of a large variety of cells with Goat Anti-Filamin reveals an extensive association of the protein with the actin containing stress fibers. Goat Anti-Filamin may be used for immunofluorescent localization of filamin in cultured cells and tissues, and to study the state of actin organization in muscle cells.
Our Abpromise guarantee covers the use of ab11074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. (PMID 18495106).|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 250 kDa.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||1/250. (see Abreview).
ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab11074 staining Filamin A in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated donkey anti-goat IgG (H+L) at a 1/500 dilution.
This image is courtesy of an Abreview submitted by Mahesh Shivananjappa, United States
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