概述

  • 产品名称Anti-FIGNL1抗体
    参阅全部 FIGNL1 一抗
  • 描述
    兔多克隆抗体to FIGNL1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human FIGNL1 (NP_071399.2)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells

性能

应用

Our Abpromise guarantee covers the use of ab70841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

Anti-FIGNL1 antibody 图像

  • All lanes : Anti-FIGNL1 antibody (ab70841) at 1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of Human FIGNL1 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab70841 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using 1 µg/mg ab70841 and chemiluminescence with an exposure time of 30 seconds.

Anti-FIGNL1 antibody (ab70841)参考文献

ab70841 has not yet been referenced specifically in any publications.

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