Anti-Fibronectin抗体[IST-9] (ab6328)

概述

  • 产品名称Anti-Fibronectin抗体[IST-9]
    参阅全部 Fibronectin 一抗
  • 描述
    小鼠单克隆抗体[IST-9] to Fibronectin
  • 特异性This antibody reacts with an epitope (PEDGIHELFP) located in the ED-A sequence of cellular fibronectin (it therefore only detects the cFN and not extracellular FN (plasma FN)). (Liao et al.) The ED-A segment can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. In transformed or tumour derived cells the ED-A segment is about 10-times higher than in FN from normal fibroblasts, and it may therefore be a significant marker for malignancy (Borsi et al., and others).
  • 经测试应用适用于: WB, RIA, ELISA, IHC-Fr, ICC/IF, Other, Blocking, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Chicken, Cow, Dog, Human, Pig, Monkey
    预测可用于: Mouse, Rabbit
  • 免疫原

    Recombinant fragment corresponding to Human Fibronectin.
    (Peptide available as ab187878)

  • 阳性对照
    • normal foetal lung fibroblasts (cell line MRC5), skin, rat or human kidney (see reviews)

性能

应用

Our Abpromise guarantee covers the use of ab6328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 mg/ml. Can be blocked with Recombinant Human Fibronectin protein (ab187878).
RIA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
ELISA 1/1500.
IHC-Fr Use at an assay dependent concentration.

Concentration of IST-9 in IHC-fr - depending on the system -  is 0.1 to 2 micrograms per ml.

IST-9 works very well using all iHC-fr procedures.

ICC/IF 1/200.
Other Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 20643910
IHC-P 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

See Abreviews

Concentration of IST-9 in IHC-P is - depending on the system - 2-10 micrograms per ml.

靶标

  • 功能Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 组织特异性Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 疾病相关Glomerulopathy with fibronectin deposits 2
  • 序列相似性Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 发展阶段Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻译后修饰Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 细胞定位Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody [IST-9] 图像

  • ab6328 staining Fibronectin in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a Tris-EDTA buffer pH9.0. Samples were incubated with primary antibody (1/50 in blocking buffer) for 30 minutes at 20°C. An undiluted HRP-conjugated Goat anti-mouse  polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Fibronectin antibody [IST-9] (ab6328) at 1/2000 dilution

    Lane 1 : Human dermal fibroblast whole cell lysate, untreated
    Lane 2 : Human dermal fibroblast whole cell lysate, treated with TGF beta 1

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated Goat anti-mouse monoclonal at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% Milk for 1 hour at 20°C.

    See Abreview

  • ab6328 staining fibronectin in human esophageal epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 1% Triton X-100 in PBS and blocked with 0.25% DAKO serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in DAKO antibody diluent) for 2 hours at 28°C. ab6785 (1/800) was used as the secondary antibody.

    This photo shows Fibronectin localization in the extracellular matrix of monolayer culture of human esophageal epithelial cells (Het-1A). Nuclei were counterstained with propidium iodide.

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  • This picture shows formalin fixed paraffin embedded rat kidney stained with ab6328 at a dilution of 1:100 after proteinase K digestion.The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
  • Ab6328 positively staining paraffin fixed canine myocardial infarct. tissue (1/200). The images demonstrate the time course of fibronectin deposition in reperfused canine myocaridal infarcts.
    a: 24 hrs
    b: 7 days
    c: 28 days of reperfusion.

    Secondary: Biotin conjugated goat anti mouse (5 µg/ml). Detection was achieved using DAB.

    This image is an edited version of an image submitted courtesy of an Abreview by Marcin Dobaczewski. We do not have any further information relating to this image.

    See Abreview

  • Anti-Fibronectin antibody [IST-9] (ab6328) at 1/1000 dilution + whole cell lysate prepared from human platelets ( treated with ADP for 30 minutes) at 20 µg

    Secondary
    HRP conjugated donkey anti-mouse polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Exposure time : 2 minutes

    Image courtesy of Dr Mahesh Shivananjappa by Abreview.

    Blocked with 5% milk for 1 hour at 22°C.

    See Abreview

Anti-Fibronectin antibody [IST-9] (ab6328)参考文献

This product has been referenced in:
  • Beach JA  et al. Sphingosine kinase 1 is required for TGF-ß mediated fibroblastto- myofibroblast differentiation in ovarian cancer. Oncotarget 7:4167-82 (2016). WB ; Human . Read more (PubMed: 26716409) »
  • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »

See all 62 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification skin
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 10 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Cardiac tissue)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Cardiac tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 21 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Chinese Hamster Cell (Chinese Hamster Ovary (CHO) cells, CHO-K1)
Specification Chinese Hamster Ovary (CHO) cells, CHO-K1
Permeabilization No
Fixative Paraformaldehyde
Username

Xander van Wijk

Verified customer

提交于 Sep 02 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Sample Mouse Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Formaldehyde
Username

Dr. Suypriya Sen

Verified customer

提交于 Aug 27 2014

The amino acid and converted RNA epitope for ab6328 are given below, along with a link to the converter tool that I used.



Amino acid sequence:

PEDGIHELFP



RNA sequence:

CCCGAAGACGGAAUUCACGAGC...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: pepsin
Sample Rabbit Tissue sections (Liver sections)
Specification Liver sections
Permeabilization No
Fixative Formaldehyde
Username

Ibrahim Badawi

Verified customer

提交于 Dec 07 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pepsin
Sample Rat Tissue sections (Liver sections)
Specification Liver sections
Permeabilization No
Fixative Formaldehyde
Username

Ibrahim Badawi

Verified customer

提交于 Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step Enzymatic
Sample Rat Tissue sections (GONAD)
Specification GONAD
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (small intestine)
Specification small intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Username

Mr. Rudolf Jung

Verified customer

提交于 Mar 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 22 2013

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