Anti-Fibronectin抗体[HFN 7.1] (ab80923)

概述

  • 产品名称
    Anti-Fibronectin抗体[HFN 7.1]
    参阅全部 Fibronectin 一抗
  • 描述
    小鼠单克隆抗体[HFN 7.1] to Fibronectin
  • 特异性
    ab80923 is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface
  • 经测试应用
    适用于: Sandwich ELISA, WB, IP, Blockingmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, Human Fibronectin, purified from serum by affinity chromatography on gelatinsepharose.

  • 表位
    Peptide core
  • 阳性对照
    • SW156 cells
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab45688 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab80923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibronectin (ab299). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Fibronectin (ab299) as Detection.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 263 kDa.
IP Use at 2 µg/mg of lysate. (Native and denatured - use Protein G)
Blocking Use at an assay dependent dilution.

靶标

  • 功能
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 组织特异性
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 疾病相关
    Glomerulopathy with fibronectin deposits 2
  • 序列相似性
    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 发展阶段
    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻译后修饰
    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 细胞定位
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

图片

文献

This product has been referenced in:
  • Ambesi A & McKeown-Longo PJ Conformational remodeling of the fibronectin matrix selectively regulates VEGF signaling. J Cell Sci 127:3805-16 (2014). Read more (PubMed: 24982443) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us.


Reducing Bovine serum will also put the cells into a lower state of growth with reduced access to growth factors. Growth factor absence will also modulate the activation state of integrin and other cell surf...

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I found a good set of products that gives a strong sandwich ELISA signal.

Capture: ab80923

Detection: ab299

Antigen: ab81743

Click here (or use the following: http://www.abcam.com/index.html?datasheet=80923)....

Read More


I've included a list of capture, detection, protein, and fluorescent-conjugated secondary below against Fibronectin. The capture and detection antibodies have been tested and guaranteed to work in sandwich ELISA to detect human fibronectin:
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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