Anti-Fibronectin抗体(Biotin) (ab6584)

概述

  • 产品名称Anti-Fibronectin抗体(Biotin)
    参阅全部 Fibronectin 一抗
  • 描述
    兔多克隆抗体to Fibronectin (Biotin)
  • 偶联物Biotin
  • 特异性negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
  • 经测试应用适用于: ICC/IF, ELISA, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
    预测可用于: all Mammals
  • 免疫原

    Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE

  • 常规说明


    This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
  • Primary antibody说明This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20686611
ELISA 1/4000 - 1/8000.
IHC-P 1/50 - 1/200.
IP Use at an assay dependent concentration.
WB 1/5000 - 1/10000.

靶标

  • 功能Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 组织特异性Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 疾病相关Glomerulopathy with fibronectin deposits 2
  • 序列相似性Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 发展阶段Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻译后修饰Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 细胞定位Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody (Biotin) 图像

  • Human Aortic Endothelial Cells grown in MCDB 131+ SmGM-2 media were seeded on a PET membrane. After one week, the cells were blocked with PBS containing 0.1% BSA for 15 min. The membrane was incubated with primary antibody (ab6584) for 30 min  and then with secondary antibody Alexa Fluor® 488 for 1 hr.

    This picture was kindly supplied as part of the review submitted by Gary Nackman and Ildiko Entersz.

  • Immunofluorescence analysis of U87-MG cells staining Fibronectin (red) with ab6584.
    Cells were treated with 10-6M Dexamethasone, before blocking and incubating with primary antibody for 1 hour at room temperature. An Alexa Fluor® 568-conjugated anti-rabbit IgG was used as the secondary antibody.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 6.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 9.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.

Anti-Fibronectin antibody (Biotin) (ab6584)参考文献

This product has been referenced in:
  • Lamichhane SP  et al. Recapitulating epithelial tumor microenvironment in vitro using three dimensional tri-culture of human epithelial, endothelial, and mesenchymal cells. BMC Cancer 16:581 (2016). IHC . Read more (PubMed: 27484993) »
  • Mia MM & Bank RA The I?B kinase inhibitor ACHP strongly attenuates TGFß1-induced myofibroblast formation and collagen synthesis. J Cell Mol Med 19:2780-92 (2015). ICC/IF ; Human . Read more (PubMed: 26337045) »

See all 12 Publications for this product

Product Wall

Thank you for your enquiry about our fibronectin antibodies. The antibody ab6584 is biotin conjugated and has been successfully used in IF (ICC) by a user of the antibody who left a review which can be accessed via the online datasheet. The reviewer di...

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For testing 1ug of antigen, the minimum of antibody used is 1:8000. The maximum is 1:4000. We don't have other data to show the sensitivity. If you have any additional questions, please contact us again.

1:4000 to 1:8000 dilution can detect 1.0ug of Fibronectin. If you have any additional questions, please contact us again.

I have not found reports of staining of fibronectin in the cytoplasm of plasma cells, however it is known that plasma cells adhere to fibronectin (the fibronectin inducing not only the adherence and spread of plasma cells but also their migration). ...

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Thank you for your enquiry. We would like to confirm that the concentration is 2.5mg/mL, so 40uL will give 100ug of antibody.

This antibody does not require antigen retrieval but it may increase staining. We normally recommend the pressure cooker method of antigen retrieval.

450 kDa non-reduced 225 kDa fully reduced

This antibody will cross-ract with mouse fibronectin using formalin fixed paraffin embedded tissues.

Yes, this antibody is available in an unconjugated form, ab299

The extracellular matrix proteins are well conserved, particularly between mammals, so human will cross-react with pig.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"