• 产品名称Anti-Fibromodulin抗体
    参阅全部 Fibromodulin 一抗
  • 描述
    兔多克隆抗体to Fibromodulin
  • 经测试应用适用于: ICC/IF, WB, IHC-Fr, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 108-157 (


    ) of human Fibromodulin (NP_002014).

  • 阳性对照
    • 721_B cell lysate



Our Abpromise guarantee covers the use of ab81443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration. PubMed: 21939397
IHC-P 1/200.
ELISA Use at an assay dependent concentration.


Anti-Fibromodulin antibody 图像

  • Anti-Fibromodulin antibody (ab81443) at 1 µg/ml + 721_B cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • ICC/IF image of ab81443 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81443, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab81443 staining Fibromodulin in Mouse aorta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% Formalin and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 10% Normal Goat serum) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/375) was used as the secondary antibody.

    See Abreview

  • Anti-Fibromodulin antibody (ab81443) at 1/500 dilution + Equine Cartilage Explants

    Predicted band size : 43 kDa

Anti-Fibromodulin antibody (ab81443)参考文献

This product has been referenced in:
  • Dyment NA  et al. The paratenon contributes to scleraxis-expressing cells during patellar tendon healing. PLoS One 8:e59944 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23555841) »
  • Yang G  et al. Enhancement of tenogenic differentiation of human adipose stem cells by tendon-derived extracellular matrix. Biomaterials 34:9295-306 (2013). WB ; Cow . Read more (PubMed: 24044998) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Heart)
Specification Heart

Abcam user community

Verified customer

提交于 Apr 18 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (HH31 wild type chick embryo trunk -sagittal sectio)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization No
Specification HH31 wild type chick embryo trunk -sagittal sectio
Blocking step BSA + 5% heat-inactivated goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1000µg/mL · Temperature: 20-25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Apr 14 2015

Thank you for that information.
As discussed, I can arrange for the ab81443 of a different lot number to be sent to you. However I will just need to check your order details for the original vial of ab81443. I have the following information, does ...

Read More

Thank you for your reply.
This has made understanding the problems you have encountered and what may be contributing to them easier to understand. I cannot be sure what is causing this problem, but from what you have described, I would suggest it ...

Read More

Thank you for contacting us and raising your concerns in regards to the anti-Fibromodulin antibody (ab81443).
I am sorry to hear you are experiencing difficulties with this product. We take product complaints very seriously, and investigate every ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (aorta)
Specification aorta
Fixative 10% formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Aug 03 2012

Expiration date: *
I am very pleased to hear you would like to accept our offer and test ab81443 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abre...

Read More