Anti-Fibrinogen抗体(Biotin) (ab34546)

概述

• 产品名称
  Anti-Fibrinogen抗体(Biotin)
  参阅全部 Fibrinogen 一抗
• 描述
  山羊多克隆抗体to Fibrinogen (Biotin)
• 偶联物
  Biotin
• 经测试应用
  适用于: ELISA, WB, Immunomicroscopymore details
• 种属反应性
  与反应: Human
  
• 免疫原

  Full length native protein (purified): Fibrinogen (Human Plasma)

• 常规说明

  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 存储溶液
  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
• Concentration information loading...
• 纯度
  IgG fraction
• 纯化说明
  This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Cardiovascular
  • Blood
  • Platelets

  • Cardiovascular
  • Blood
  • Coagulation
  • Common

  • Cardiovascular
  • Atherosclerosis
  • Thrombosis
  • Other

  • Metabolism
  • Types of disease
  • Metabolic disorders

相关产品

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)

应用

靶标

• 功能
  Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
• 组织特异性
  Plasma.
• 疾病相关
  Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
  Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
• 序列相似性
  Contains 1 fibrinogen C-terminal domain.
• 结构域
  A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
• 翻译后修饰
  The alpha chain is not glycosylated.
  Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
  About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
  Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
  Phosphorylation sites are present in the extracellular medium.
• 细胞定位
  Secreted.

• Target information above from: UniProt accession P02671 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 2243 Human
  • Entrez Gene: 2244 Human
  • Entrez Gene: 2266 Human
  • Omim: 134820 Human
  • Omim: 134830 Human
  • Omim: 134850 Human
  • SwissProt: P02671 Human
  • SwissProt: P02675 Human

  • SwissProt: P02679 Human
  • Unigene: 300774 Human
  • Unigene: 351593 Human
  • Unigene: 727584 Human

  see all
• 别名
  • FGA antibody
  • Fib2 antibody
  • FIBA_HUMAN antibody

  • Fibrinogen A alpha polypeptide antibody
  • Fibrinogen alpha chain antibody
  • Fibrinogen B alpha polypeptide antibody
  • Fibrinogen beta chain antibody
  • Fibrinogen G alpha polypeptide antibody
  • Fibrinogen gamma chain antibody
  • fibrinogen, B beta polypeptide antibody
  • fibrinogen, G gamma polypeptide antibody
  • fibrinogen, gamma polypeptide antibody
  • Fibrinogen--alpha -polypeptide chain antibody
  • Fibrinogen--beta -polypeptide chain antibody
  • Fibrinogen--gamma-polypeptide chain antibody

  see all

实验方案

• Western blot protocols