Anti-Fibrinogen抗体(ab34269)
Key features and details
- Rabbit polyclonal to Fibrinogen
- Suitable for: IHC-P, ICC/IF, Functional Studies, Sandwich ELISA
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Fibrinogen抗体
参阅全部 Fibrinogen 一抗 -
描述
兔多克隆抗体to Fibrinogen -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, Functional Studies, Sandwich ELISAmore details -
种属反应性
与反应: Mouse, Rabbit, Human
预测可用于: Rat -
免疫原
Full length native protein (purified) corresponding to Human Fibrinogen.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (8) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first. |
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Sandwich ELISA |
1/200.
For sandwich ELISA, use this antibody as Detection at 1/200 dilution with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) as Capture. |
说明 |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first. |
Sandwich ELISA
1/200. For sandwich ELISA, use this antibody as Detection at 1/200 dilution with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) as Capture. |
靶标
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功能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
组织特异性
Plasma. -
疾病相关
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
结构域
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻译后修饰
The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2243 Human
- Entrez Gene: 2244 Human
- Entrez Gene: 2266 Human
- Entrez Gene: 110135 Mouse
- Entrez Gene: 14161 Mouse
- Entrez Gene: 99571 Mouse
- Entrez Gene: 24366 Rat
- Entrez Gene: 24367 Rat
see all -
别名
- FGA antibody
- FGB antibody
- FGG antibody
see all
图片
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Ab34269 staining human liver. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1/50. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Standard Curve for Fibrinogen (Analyte: Fibrinogen protein (Human) (ab84410)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) at 5µg/ml and Detector Antibody Rabbit polyclonal to Fibrinogen (ab34269) at 1/2000.
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ab34269 staining Fibrinogen in Human liver/placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer, pH 6.0 for 20 minutes at 100°C. The primary antibody was diluted 1/2000 and incubated with the sample for 20 minutes at 25°C. An HRP polymer-conjugated mouse anti-rabbit polyclonal IgG was used as the secondary antibody.
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Immunohistochemical analysis of rat heart, staining Fibrinogen with ab34269.
Tissue was fixed with formaldehyde and blocked with 0.25% BSA for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1.5 µg/ml in 1% BSA in TBS) for 20 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
实验方案
数据表及文件
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Datasheet download
文献 (55)
ab34269 被引用在 55 文献中.
- Georgiadou A et al. Comparative transcriptomic analysis reveals translationally relevant processes in mouse models of malaria. Elife 11:N/A (2022). PubMed: 35006075
- Pei S et al. Lysophosphatidic Acid Receptor 3 Suppress Neutrophil Extracellular Traps Production and Thrombosis During Sepsis. Front Immunol 13:844781 (2022). PubMed: 35464399
- Ghorbani S et al. Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination. Nat Commun 13:2445 (2022). PubMed: 35508608
- Sun YE et al. Different Thrombus Histology in a Cancer Patient with Deep Vein Thrombosis and Recurrent Strokes. J Stroke 24:300-302 (2022). PubMed: 35677986
- Zhao C et al. Delayed administration of nafamostat mesylate inhibits thrombin-mediated blood-spinal cord barrier breakdown during acute spinal cord injury in rats. J Neuroinflammation 19:189 (2022). PubMed: 35842640