概述

  • 产品名称Anti-Fibrinogen抗体
    参阅全部 Fibrinogen 一抗
  • 描述
    兔多克隆抗体to Fibrinogen
  • 特异性Ab27913 recognises fibrinogen.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Full length native protein (purified): Mouse fibrinogen isolated from plasma using several chromatographic steps.

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Liver.

性能

应用

Our Abpromise guarantee covers the use of ab27913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Predicted molecular weight: 95 kDa.
ELISA 1/100000.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • 组织特异性Plasma.
  • 疾病相关Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 序列相似性Contains 1 fibrinogen C-terminal domain.
  • 结构域A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • 翻译后修饰The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FGA antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen A alpha polypeptide antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen B alpha polypeptide antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen G alpha polypeptide antibody
    • Fibrinogen gamma chain antibody
    • fibrinogen, B beta polypeptide antibody
    • fibrinogen, G gamma polypeptide antibody
    • fibrinogen, gamma polypeptide antibody
    • Fibrinogen--alpha -polypeptide chain antibody
    • Fibrinogen--beta -polypeptide chain antibody
    • Fibrinogen--gamma-polypeptide chain antibody
    see all

Anti-Fibrinogen antibody 图像

  • IHC image of Fibrinogen staining in Mouse Liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27913, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Fibrinogen antibody (ab27913)参考文献

This product has been referenced in:
  • Nordstrom SM  et al. Increased thrombosis susceptibility and altered fibrin formation in STAT5-deficient mice. Blood 116:5724-33 (2010). WB ; Mouse . Read more (PubMed: 20823455) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 26 2015

Thank you for contacting us.

This antibody is not able to detect the individual subunits of fibrinogen.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informati...

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Thank you for contacting Abcam regarding ab27913.


This antibody is expected to react with fibrin as it was made against full length fibrinogen. Unfortunately, wedo not have a supporting western to provide.


I hope this inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"