Anti-Fibrinogen alpha chain抗体[UC45] (ab19079)

概述

  • 产品名称
    Anti-Fibrinogen alpha chain抗体[UC45]
    参阅全部 Fibrinogen alpha chain 一抗
  • 描述
    小鼠单克隆抗体[UC45] to Fibrinogen alpha chain
  • 特异性
    This antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted.
  • 经测试应用
    适用于: ICC, Flow Cyt, RIA, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Human acute monoblastic leukemia cells.

  • 常规说明


    A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.

性能

应用

Our Abpromise guarantee covers the use of ab19079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
RIA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. PubMed: 24959311

靶标

  • 功能
    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • 组织特异性
    Plasma.
  • 疾病相关
    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 序列相似性
    Contains 1 fibrinogen C-terminal domain.
  • 结构域
    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • 翻译后修饰
    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ac1873 antibody
    • Fba5e antibody
    • FGA antibody
    • Fib antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen, A alpha polypeptide antibody
    • Fibrinogen--alpha polypeptide chain antibody
    • Fibrinopeptide A antibody
    see all

图片

  • Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Richens JL  et al. Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid. Int J Mol Epidemiol Genet 5:53-70 (2014). ELISA ; Human . Read more (PubMed: 24959311) »
  • Wu X  et al. Molecular MRI of atherosclerotic plaque progression in an ApoE(-/-) mouse model with a CLT1 peptide targeted macrocyclic Gd(III) chelate. Am J Nucl Med Mol Imaging 3:446-55 (2013). Read more (PubMed: 24116353) »

See all 5 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (The Mouse Arteriovenous Fistula Model)
Antigen retrieval step
None
Permeabilization
No
Specification
The Mouse Arteriovenous Fistula Model
Blocking step
0.4% Casein (in phosphate-buffered saline with stabilizers, surfactant, 0.2% Bronidox L as a preservative) as blocking agent for 10 minute(s) · Concentration: 0.4% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Jia Hong Lin

Verified customer

提交于 Dec 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (islet cell)
Specification
islet cell
Fixative
frozen (OCT)
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 20 2012

Thank you for your enquiry regarding ab19079 and your interest in our products. This antibody reacts very strongly with the alpha chain of human fibrinogen – for further information please see Hogg 1983 paper quoted. I hope this information is us...

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Thank you for your enquiry. Following correspondence with the source of this antibody I have determined that this antibody was applied using immunocytochemistry in the following publication: Hogg N Human monocytes are associated with the formati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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