Anti-Fibrinogen alpha chain抗体[EPR2919] (ab92572)


  • 产品名称Anti-Fibrinogen alpha chain抗体[EPR2919]
    参阅全部 Fibrinogen alpha chain 一抗
  • 描述
    兔单克隆抗体[EPR2919] to Fibrinogen alpha chain
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    corresponding to Human Fibrinogen alpha chain aa 750-850.
    Database link: P02671

  • 阳性对照
    • Human hepatocellular carcinoma tissue Human plasma, fetal liver and HepG2 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab92572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP 1/50.
IHC-P 1/50 - 1/100.

Antigen retrieval plus the use of an HRP/AP polymerized secondary antibody is highly recommended for enhanced staining.

ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • 组织特异性Plasma.
    • 疾病相关Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
      Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • 序列相似性Contains 1 fibrinogen C-terminal domain.
    • 结构域A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • 翻译后修饰The alpha chain is not glycosylated.
      Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
      About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
      Phosphorylation sites are present in the extracelllular medium.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ac1873 antibody
      • Fba5e antibody
      • FGA antibody
      • Fib antibody
      • FIBA_HUMAN antibody
      • Fibrinogen, A alpha polypeptide antibody
      • Fibrinopeptide A antibody
      see all

    Anti-Fibrinogen alpha chain antibody [EPR2919] 图像

    • All lanes : Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572) at 1/2000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 95 kDa
    • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab92572 at 1/2000 dilution.

    Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)参考文献

    ab92572 has not yet been referenced specifically in any publications.

    Product Wall

    The immunogen sequence used to generate ab92572 is a short peptide found with Fibrinogen alpha chain. It does not specifically detect the Fibrinopeptide A molecule.

    Thank you for your enquiry. The immunogen contains Fibrinogen alpha chain and is in the Fibrinogen C-terminal region. This antibody detects the main protein Fibrinogen. If Fibrinopeptide A has not been cleaved from Fibrinogen alpha chain, then ...

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