antigen sequence: TLHHYFVPDG DYEENDDPEK CQLLFRVSDH RRCSQGEGSQ VGSLLSLTLR EEFTVLGRQV EDAGRVLEGI SKSISYD, corresponding to amino acids 32-108 of Human FIBIN.
- FIBIN over-expression HEK293T Lysate; Human placenta tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the FIBIN family.
Secreted. Golgi apparatus.
Information by UniProt
- Fibin antibody
- FIBIN_HUMAN antibody
- Fin bud initiation factor homolog (zebrafish) antibody
Immunocytochemistry/ Immunofluorescence - Anti-FIBIN antibody (ab122621)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122621 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIBIN antibody (ab122621)
ab122621 at 1/50 dilution staining FIBIN in paraffin-embedded Human placenta tissue by Immunohistochemistry.
Western blot - Anti-FIBIN antibody (ab122621)
All lanes : Anti-FIBIN antibody (ab122621) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : FIBIN Over-expression HEK293T Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
has not yet been referenced specifically in any publications.
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