概述

  • 产品名称
  • 描述
    兔多克隆抗体to FHOD3
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 390 - 439 (SGKFSGSSPA PPSQPQGLSY AEDAAEHENM KAVLKTSSPS VEDATPALGV) of Human FHOD3 (AAH81563).

  • 阳性对照
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue

性能

应用

Our Abpromise guarantee covers the use of ab105651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Actin-organizing protein that may cause stress fiber formation together with cell elongation.
  • 组织特异性
    Expressed in the heart, kidney and brain.
  • 序列相似性
    Belongs to the formin homology family.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 细胞定位
    Cytoplasm > cytoskeleton. Main part of the protein localizes to actin fibers and the remaining part displays filamentous staining.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ22717 antibody
    • Formactin2 antibody
    • FH1/FH2 domain containing protein 3 antibody
    • FH1/FH2 domain-containing protein 3 antibody
    • Fhod3 antibody
    • FHOD3_HUMAN antibody
    • FHOS2 antibody
    • FLJ22297 antibody
    • Formactin-2 antibody
    • Formin homolog overexpressed in spleen 2 antibody
    • Formin homology 2 domain containing 3 antibody
    • hFHOS2 antibody
    • KIAA1695 antibody
    • mKIAA1695 antibody
    see all

Anti-FHOD3 antibody 图像

  • Anti-FHOD3 antibody (ab105651) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 60 kDa
    The band at 60kD may be either a non-specific protein or a degradation product of the larger ~155-160kD FHOD3 protein. There are no known cleavage products or isoforms known to be around this size. Based on the peptide immunogen used to raise this antibody, this band may be isoform 2 of human CARD14 (434aa; ~82% homology).

    Gel concetration: 12%
  • ab105651, at 8 µg/ml, staining FHOD3 in paraffin embeded Human kidney tissue by Immunohistochemistry. Cells with positive label are the epithelial cells from renal tubules (indicated by arrows). Magnification: 400X.

Anti-FHOD3 antibody (ab105651)参考文献

This product has been referenced in:
  • Lynch J  et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . Read more (PubMed: 24223803) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Embryonic Heart Cryo-Section)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart Cryo-Section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jul 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4%-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 25 2016

Thank you for contacting us.

This band at ˜60kDa has been seen using this product and is on our website (http://www.abcam.com/FHOD3-antibody-ab105651.html#FHOD3-Primary-antibodies-ab105651-1.jpg).The band at 60kD may be either a non-specific...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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