概述

  • 产品名称Anti-FHOD1抗体
    参阅全部 FHOD1 一抗
  • 描述
    小鼠多克隆抗体to FHOD1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    full-length human FHOD1 protein (NP_037373.2, 1 a.a. ~ 1164 a.a).

  • 阳性对照
    • K-562 cell lysate and FHOD1 transfected 293T cell lysate human spleen

性能

应用

Our Abpromise guarantee covers the use of ab73443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 127 kDa.
IHC-P Use a concentration of 13 µg/ml.

靶标

  • 功能Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.
  • 组织特异性Ubiquitous. Highly expressed in spleen.
  • 序列相似性Belongs to the formin homology family.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 结构域Regulated by intramolecular binding to a C-terminal auto-inhibitory domain. Effector binding abolishes this interaction and activates the protein.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FH1/FH2 domain containing protein antibody
    • FH1/FH2 domain containing protein 1 antibody
    • FH1/FH2 domain-containing protein 1 antibody
    • Fhod1 antibody
    • FHOD1_HUMAN antibody
    • FHOS antibody
    • FHOS1 antibody
    • Formin homolog overexpressed in spleen 1 antibody
    • Formin homology 2 domain containing 1 antibody
    • Formin homology 2 domain containing protein 1 antibody
    • Formin homology 2 domain-containing protein 1 antibody
    see all

Anti-FHOD1 antibody 图像

  • All lanes : Anti-FHOD1 antibody (ab73443) at 1/500 dilution

    Lane 1 : FHOD1 transfected 293T cell lysate
    Lane 2 : non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 127 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • Anti-FHOD1 antibody (ab73443) at 1/500 dilution + K-562 cell lysate at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 127 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.

Anti-FHOD1 antibody (ab73443)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab73443.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"