ab56068 detects FHL2. It also binds FHL2 isoform 5 with MW of 389 amino acids (~42kDa).
Synthetic peptide derived from the human FHL2 protein
- Antibody tested on HeLa Lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.08% Sodium Azide
Concentration information loading...
Purified by SAS precipitation.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
Expressed in skeletal muscle and heart.
Contains 4 LIM zinc-binding domains.
The third LIM zinc-binding mediates interaction with E4F1.
Information by UniProt
- AAG 11 antibody
- AAG11 antibody
- Aging associated gene 11 antibody
Western blot - FHL2 antibody (ab56068)
Lanes 1-2 :
Anti-FHL2 antibody (ab56068) at 0.4 µg/mlLane 1 :
HeLa cell extract at 10ug/laneLane 2 :
HeLa cell extract at 10ug/lane with 16 ug blocking peptideLane 3 :
HeLa cell extract at 10ug/lane. Goat anti Rabbit HRP controlSecondaryAll lanes :
goat anti-rabbit Ig HRP at 1/75000 dilution
Developed using the ECL technique.Predicted band size:
32 kDaObserved band size:
34 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
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