概述

  • 产品名称
  • 描述
    兔多克隆抗体to FHL2
  • 特异性
    ab56068 detects FHL2. It also binds FHL2 isoform 5 with MW of 389 amino acids (~42kDa).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the human FHL2 protein

  • 阳性对照
    • Antibody tested on HeLa Lysates

性能

应用

Our Abpromise guarantee covers the use of ab56068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).

靶标

  • 功能
    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • 组织特异性
    Expressed in skeletal muscle and heart.
  • 序列相似性
    Contains 4 LIM zinc-binding domains.
  • 结构域
    The third LIM zinc-binding mediates interaction with E4F1.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

图片

  • Lanes 1 - 2 : Anti-FHL2 antibody (ab56068) at 0.4 µg/ml
    Lane 3 :

    Lane 1 : HeLa cell extract at 10ug/lane
    Lane 2 : HeLa cell extract at 10ug/lane with 16 ug blocking peptide
    Lane 3 : HeLa cell extract at 10ug/lane. Goat anti Rabbit HRP control

    Secondary
    goat anti-rabbit Ig HRP at 1/75000 dilution
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab56068 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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