Anti-FHL2抗体(ab137477)
Key features and details
- Rabbit polyclonal to FHL2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-FHL2抗体
参阅全部 FHL2 一抗 -
描述
兔多克隆抗体to FHL2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow -
免疫原
Recombinant fragment corresponding to a region within amino acids 52-272 of Human FHL2 (UniProt ID: Q14192).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137477于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
靶标
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功能
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. -
组织特异性
Expressed in skeletal muscle and heart. -
序列相似性
Contains 4 LIM zinc-binding domains. -
结构域
The third LIM zinc-binding mediates interaction with E4F1. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 510008 Cow
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Entrez Gene: 63839 Rat
- Omim: 602633 Human
- SwissProt: Q2KI95 Cow
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
see all -
别名
- AAG 11 antibody
- AAG11 antibody
- Aging associated gene 11 antibody
see all
图片
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Anti-FHL2 antibody (ab137477) at 1/1500 dilution + Pig aortic valve interstitial cells lysate at 2.5 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG (H+L) polyclonal at 1/4500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 5 minutes
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Anti-FHL2 antibody (ab137477) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 32 kDa
12% SDS PAGE -
Anti-FHL2 antibody (ab137477) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg
Predicted band size: 32 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin embedded DLD1 xenograft labelling FHL2 with ab137477 at a 1/100 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab137477 尚未被引用在任何文献中。