概述

  • 产品名称Anti-FHL2抗体
    参阅全部 FHL2 一抗
  • 描述
    兔多克隆抗体to FHL2
  • 特异性Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A synthetic peptide which represented a portion of human FHL2 encoded within exon 7.

  • 阳性对照
    • HeLa nuclear extract.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is affinity purified.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ICC/IF 1/100.

靶标

  • 功能May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • 组织特异性Expressed in skeletal muscle and heart.
  • 序列相似性Contains 4 LIM zinc-binding domains.
  • 结构域The third LIM zinc-binding mediates interaction with E4F1.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

Anti-FHL2 antibody 图像



  • Predicted band size : 32 kDa


    Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
  • ICC/IF image of ab12327 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FHL2 antibody (ab12327)参考文献

This product has been referenced in:
  • Friedrich FW  et al. FHL2 expression and variants in hypertrophic cardiomyopathy. Basic Res Cardiol 109:451 (2014). Read more (PubMed: 25358972) »
  • Gökmen-Polar Y  et al. Dual targeting of EphA2 and ER restores tamoxifen sensitivity in ER/EphA2-positive breast cancer. Breast Cancer Res Treat 127:375-84 (2011). WB ; Human . Read more (PubMed: 20602165) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Permeabilization Yes - saponin
Specification Human pluripotent stem cell derived cardiomyocyte
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 21 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Mouse Cell lysate - whole cell (bone marrow cells)
Specification bone marrow cells
Treatment 30 ng/ml RANKL and 30 ng/ml MCSF for 5 days
Total protein in input 2000 µg
Username

Do Yu Soung

Verified customer

提交于 Jul 26 2013

Le peptide bloquant de l'anti-FHL2 ab12327 est maintenant disponible à la vente sous la référence ab119360. La fiche technique est disponible via le lien www.abcam.com/ab119360 . J'espère que ces informations vous seront utiles. N'hésitez pas à nous...

Read More

Le laboratoire m'a informé que le peptide bloquant est disponible à la vente, il sera ajouté au catalogue dans la journée d'aujourd'hui ou au plus tard demain. Je vous enverrai la référence de ce produit quand la fiche technique aura été créée. La s...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (osteoblast)
Specification osteoblast
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"