• 产品名称Anti-FHIT抗体
    参阅全部 FHIT 一抗
  • 描述
    兔多克隆抗体to FHIT
  • 特异性FHIT antibody detects endogenous levels of total FHIT protein.
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from human FHIT

  • 阳性对照
    • Human breast carcinoma; A549 cell extract.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab53074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.


  • 相关性FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • AP3A hydrolase antibody
    • AP3A hydrolase fragile site 3p14.2 antibody
    • AP3Aase antibody
    • Bis 5' adenosyl triphosphatase antibody
    • Bis(5' adenosyl) triphosphatase antibody
    • Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody
    • Dinucleosidetriphosphatase antibody
    • FRA 3B antibody
    • FRA3B antibody
    • Fragile histidine triad antibody
    • Fragile histidine triad gene antibody
    • Fragile histidine triad protein antibody
    • Tumor suppressor protein antibody
    see all

Anti-FHIT antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
    using ab53074 at 1/50 dilution, with and without peptide immunogen.
  • All lanes : Anti-FHIT antibody (ab53074) at 1/500 dilution

    Lane 1 : A549 cell extract with no immunizing peptide
    Lane 2 : A549 cell extract with immunizing peptide

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
  • ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FHIT antibody (ab53074)参考文献

ab53074 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I am pleased to provide the testing protocol below, including the antigen retreival. I hope this will be helpful. Please note this is a guideline only, and may require some further individual optimization by the end...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (lymphocyte)
Specification lymphocyte
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. Julian Laubental

Verified customer

提交于 Apr 20 2012

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

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Thank you for your patient. The immunogen peptide sequence range is amino acids 110-130. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.