Anti-FH/Fumarase抗体(ab95950)
Key features and details
- Rabbit polyclonal to FH/Fumarase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FH/Fumarase抗体
参阅全部 FH/Fumarase 一抗 -
描述
兔多克隆抗体to FH/Fumarase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Zebrafish -
免疫原
Recombinant fragment corresponding to Human FH/Fumarase aa 89-335.
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阳性对照
- 293T and A431 whole cell lysates OVCAR3 xenograft tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/500. |
靶标
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功能
Also acts as a tumor suppressor. -
通路
Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. -
疾病相关
Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839]. -
序列相似性
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. -
细胞定位
Cytoplasm and Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 520260 Cow
- Entrez Gene: 2271 Human
- Entrez Gene: 14194 Mouse
- Entrez Gene: 24368 Rat
- Entrez Gene: 393938 Zebrafish
- Omim: 136850 Human
- SwissProt: P07954 Human
- SwissProt: P97807 Mouse
see all -
别名
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
see all
图片
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All lanes : Anti-FH/Fumarase antibody (ab95950) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa -
ab95950 at a 1:500 dilution, staining human FH/Fumarase in OVCAR3 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab95950 被引用在 3 文献中.
- Sarkar P et al. Reduced expression of mitochondrial fumarate hydratase in progressive multiple sclerosis contributes to impaired in vitro mesenchymal stromal cell-mediated neuroprotection. Mult Scler 28:1179-1188 (2022). PubMed: 34841955
- Ueno D et al. Targeting Krebs-cycle-deficient renal cell carcinoma with Poly ADP-ribose polymerase inhibitors and low-dose alkylating chemotherapy. Oncotarget 13:1054-1067 (2022). PubMed: 36128328
- Zacharias NM et al. Prolyl Hydroxylase 3 Knockdown Accelerates VHL-Mutant Kidney Cancer Growth In Vivo. Int J Mol Sci 22:N/A (2021). PubMed: 33799686