概述
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产品名称Anti-FGR抗体[EPR3170]
参阅全部 FGR 一抗 -
描述兔单克隆抗体[EPR3170] to FGR
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经测试应用适用于: WB, IPmore details
不适用于: Flow Cyt,ICC or IHC-P -
种属反应性与反应: Human
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免疫原
Synthetic peptide (Human) corresponding to residues in Human FGR
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阳性对照
- HL-60 cell lysate treated with retinoic acid Raji cell lysate PBMC cell lysate
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常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR3170
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同种型IgG
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研究领域
相关产品
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab92302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IP |
应用说明
IP: 1/10.
WB: 1/1000 - 1/2000. Predicted molecular weight: 59 kDa.
Is unsuitable for Flow Cyt, ICC or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1.
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组织特异性Detected in neutrophils, monocytes and natural killer cells (at protein level). Detected in monocytes and large lymphocytes.
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疾病相关Mutations that cause aberrant kinase activation can confer oncogene activity and promote aberrant cell proliferation.
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序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling; this does not lead to degradation.
Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412. -
细胞定位Cell membrane. Cell membrane. Cell projection > ruffle membrane. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mitochondrion inner membrane. Mitochondrion intermembrane space. Detected in mitochondrial intermembrane space and at inner membranes (By similarity). Colocalizes with actin fibers at membrane ruffles. Detected at plasma membrane lipid rafts.
- Information by UniProt
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数据库链接
- Entrez Gene: 2268 Human
- NCBI: 4885235 Human
- Omim: 164940 Human
- SwissProt: P09769 Human
- Unigene: 1422 Human
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别名
- c fgr antibody
- c fgr protooncogene antibody
- c src 2 proto oncogene antibody
see all
Anti-FGR antibody [EPR3170] 图像
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![Western blot - FGR antibody [EPR3170] (ab92302)](http://www.abcam.cn/ps/datasheet/images/92/ab92302/FGR-Primary-antibodies-ab92302-5.jpg)
Western blot - FGR antibody [EPR3170] (ab92302)All lanes : Anti-FGR antibody [EPR3170] (ab92302) at 1/1000 dilution
Lane 1 : HL-60 cell lysate treated with retinoic acid
Lane 2 : Raji cell lysate
Lane 3 : PBMC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 59 kDa
Anti-FGR antibody [EPR3170] (ab92302)参考文献
ab92302 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"