Anti-FGFR4抗体(ab5481)

概述

  • 产品名称Anti-FGFR4抗体
    参阅全部 FGFR4 一抗
  • 描述
    兔多克隆抗体to FGFR4
  • 经测试应用适用于: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human FGFR4 aa 24-55 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • WB: SH-SY5Y, 293 and Raji cell lysates. ICC/IF: HeLa cells. IHC-P: Human hepatocarcinoma tissue. FC: WiDr cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Primary antibody说明Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab5481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
  • 组织特异性Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Glycosylated.
    Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
  • 细胞定位Membrane. Isoform 2 may be secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 334 antibody
    • CD334 antibody
    • CD334 antigen antibody
    • fc13h 10 antibody
    • fc13h10 antibody
    • Fgfr 4 antibody
    • FGFR-4 antibody
    • Fgfr4 antibody
    • FGFR4_HUMAN antibody
    • Fibroblast growth factor receptor 4 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 2 antibody
    • JTK2 antibody
    • MGC20292 antibody
    • Protein tyrosine kinase antibody
    • TKF antibody
    • Tyrosine kinase related to fibroblast growth factor receptor antibody
    • Tyrosylprotein kinase antibody
    see all

Anti-FGFR4 antibody 图像

  • All lanes : Anti-FGFR4 antibody (ab5481) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 87 kDa
  • Anti-FGFR4 antibody (ab5481) + 293 cell lysate at 35 µg

    Predicted band size : 87 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FGFR4 with ab5481 at 1/200. Cells were fixed with 4% paraformaldehyde for 20 minutes and permeabilized with 0.2% Triton X-100 for 30 minutes. Cells were incubated with the primary antibody for 2 hours at room temperature. An Alexa Fluor® 488 conjugated donkey anti-rabbit IgG (green) was used as the secondary antibody (1/1000, 1 hour). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 minutes).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling FGFR4 with ab5481. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

  • Flow cytometry analysis of WiDr cells labelling FGFR4 (green) with ab5481 compared to a negative control. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-FGFR4 antibody (ab5481)参考文献

ab5481 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human fetal heart)
Specification human fetal heart
Fixative Paraformaldehyde
Permeabilization Yes - saponin 0.1%
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
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Abcam user community

Verified customer

提交于 Mar 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549 cells)
Loading amount 10 µg
Specification A549 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 27 2009

Thank you for your enquiry. Further to correspondence with the source of this antibody unfortunately I have been unable to determine whether the multiple bands that you have been observing is typical of any of these antibodies. As a general rule...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"