概述

  • 产品名称Anti-FGFR4抗体
    参阅全部 FGFR4 一抗
  • 描述
    兔多克隆抗体to FGFR4
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human FGFR4.

    (Peptide available as ab42267.)

  • 阳性对照
    • ab41948 gave a positive result in MDA MB 361 whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human liver cancer.

性能

应用

Our Abpromise guarantee covers the use of ab41948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
  • 组织特异性Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Glycosylated.
    Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
  • 细胞定位Membrane. Isoform 2 may be secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 334 antibody
    • CD334 antibody
    • CD334 antigen antibody
    • fc13h 10 antibody
    • fc13h10 antibody
    • Fgfr 4 antibody
    • FGFR-4 antibody
    • Fgfr4 antibody
    • FGFR4_HUMAN antibody
    • Fibroblast growth factor receptor 4 antibody
    • Hydroxyaryl protein kinase antibody
    • JTK 2 antibody
    • JTK2 antibody
    • MGC20292 antibody
    • Protein tyrosine kinase antibody
    • TKF antibody
    • Tyrosine kinase related to fibroblast growth factor receptor antibody
    • Tyrosylprotein kinase antibody
    see all

Anti-FGFR4 antibody 图像

  • Anti-FGFR4 antibody (ab41948) at 1 µg/ml + MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab41948 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41948, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.

  • IHC image of FGFR4 staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41948, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-FGFR4 antibody (ab41948)参考文献

This product has been referenced in:
  • Page RL  et al. Induction of stem cell gene expression in adult human fibroblasts without transgenes. Cloning Stem Cells 11:417-26 (2009). ICC/IF ; Human . Read more (PubMed: 19622035) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549)
Loading amount 10 µg
Specification A549
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 27 2009

Application Western blot
Sample Mouse Tissue lysate - whole (Lung, Heart, Skeletal muscle)
Loading amount 20 µg
Specification Lung, Heart, Skeletal muscle
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jun 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"