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Our Abpromise guarantee covers the use of ab89476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/1000. Predicted molecular weight: 92 kDa.|
|IHC-P||1/20 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. The recommended enzyme for this clone is Proteinase K (20ug/ml in PBS, incubated at room temperature for 15-30 minutes.|
|Neutralising||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"