概述

  • 产品名称Anti-FGFR2抗体
    参阅全部 FGFR2 一抗
  • 描述
    兔多克隆抗体to FGFR2
  • 特异性FGFR2 extracellular reacts specifically with FGFR2 using immunoblotting and immunoprecipitation (doublet at 115-125kDa) using whole cell lysates of transfected 293T human, embryonic kidney cells expressing recombinant human FGFR2. No reaction with human FGFR1 and FGFR3 is detected.
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    APGREKEITASPDK

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 362-374 of Human FGFR2.

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab10648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 110 kDa. 1/2000 this dilution is determined by blotting using a whole extract of transfected cells expressing recombinant human FGFR2. Predicted molecular weight: 110 kDa.
IP 1/1000. 1/1000, this dilution is determined by immunoprecipitation using a whole lysate of transfected cells expressing recombinant human FGFR2.
IHC-P 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. The recommended enzyme is trypsin.

靶标

  • 功能Receptor for acidic and basic fibroblast growth factors.
  • 疾病相关Defects in FGFR2 are the cause of Crouzon syndrome (CS) [MIM:123500]; also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism.
    Defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS) [MIM:123150]. JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet: broad great toes with medial deviation and tarsal-metatarsal coalescence.
    Defects in FGFR2 are a cause of Apert syndrome (APRS) [MIM:101200]; also known as acrocephalosyndactyly type 1 (ACS1). APRS is a syndrome characterized by facio-cranio-synostosis, osseous and membranous syndactyly of the four extremities, and midface hypoplasia. The craniosynostosis is bicoronal and results in acrocephaly of brachysphenocephalic type. Syndactyly of the fingers and toes may be total (mitten hands and sock feet) or partial affecting the second, third, and fourth digits. Intellectual deficit is frequent and often severe, usually being associated with cerebral malformations.
    Defects in FGFR2 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of Pfeiffer syndrome have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2); craniosynostosis, early demise, sporadic (type 3).
    Defects in FGFR2 are the cause of Beare-Stevenson cutis gyrata syndrome (BSCGS) [MIM:123790]. BSCGS is an autosomal dominant condition is characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death.
    Defects in FGFR2 are the cause of familial scaphocephaly syndrome (FSPC) [MIM:609579]; also known as scaphocephaly with maxillary retrusion and mental retardation. FSPC is an autosomal dominant craniosynostosis syndrome characterized by scaphocephaly, macrocephaly, hypertelorism, maxillary retrusion, and mild intellectual disability. Scaphocephaly is the most common of the craniosynostosis conditions and is characterized by a long, narrow head. It is due to premature fusion of the sagittal suture or from external deformation.
    Defects in FGFR2 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
    Defects in FGFR2 are the cause of Antley-Bixler syndrome (ABS) [MIM:207410]. ABS is a multiple congenital anomaly syndrome characterized by craniosynostosis, radiohumeral synostosis, midface hypoplasia, malformed ears, arachnodactyly and multiple joint contractures. ABS is a heterogeneous disorder and occurs with and without abnormal genitalia in both sexes.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bacteria-expressed kinase antibody
    • BBDS antibody
    • BEK antibody
    • BEK fibroblast growth factor receptor antibody
    • BFR1 antibody
    • CD332 antibody
    • CD332 antigen antibody
    • CEK3 antibody
    • CFD1 antibody
    • Craniofacial dysostosis 1 antibody
    • ECT1 antibody
    • FGF receptor antibody
    • FGFR 2 antibody
    • FGFR-2 antibody
    • Fgfr2 antibody
    • FGFR2_HUMAN antibody
    • Fibroblast growth factor receptor 2 antibody
    • Hydroxyaryl protein kinase antibody
    • Jackson Weiss syndrome antibody
    • JWS antibody
    • K SAM antibody
    • K-sam antibody
    • Keratinocyte growth factor receptor 2 antibody
    • Keratinocyte growth factor receptor antibody
    • KGFR antibody
    • KSAM antibody
    • protein tyrosine kinase, receptor like 14 antibody
    • soluble FGFR4 variant 4 antibody
    • TK14 antibody
    • TK25 antibody
    see all

Anti-FGFR2 antibody 图像

  • ab10648 staining FGFR2 in the epithelium (top) and transition zone (bottom) of Embryonic Mouse eye tissue sections by Immunohistochemistry ((IHC) paraffin-embedded sections). Tissue was fixed, embedded in paraffin and sectioned. Sections were trypsinized for 40 minutes at room temperature in a humidified chamber, washed in PBS, and then incubated for 1 hour with a 0.5% Triton X-100 and 0.3 M glycine in PBS in a humidified chamber. After antigen retrieval, sections were blocked in 0.5% nonfat dry milk, 10% horse serum, and 0.2% Triton X-100 diluted in PBS for 3 hours at room temperature. An Alexa Fluor®568-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded analysis of hb/hb and control mouse embryo (P5) tissue sections labelling FGFR2 with ab10648. Samples were fixed overnight at 4°C in 10% neutral-buffered formalin, embedded in paraffin and cut into 8µm sections.

    At this stage, FGFR2 is located in hair cells (black arrowheads) and tectorial membrane (red arrowhead). No significant differences in the Fgfr2 protein levels are detected in hb/hb mutants compared to controls. Scale bar: 10µm.

Anti-FGFR2 antibody (ab10648)参考文献

This product has been referenced in:
  • Lee S & Griep AE Loss of Dlg-1 in the mouse lens impairs fibroblast growth factor receptor signaling. PLoS One 9:e97470 (2014). ICC/IF ; Mouse . Read more (PubMed: 24824078) »
  • Huang Y  et al. Twist1- and twist2-haploinsufficiency results in reduced bone formation. PLoS One 9:e99331 (2014). IHC-P ; Mouse . Read more (PubMed: 24971743) »

See all 13 Publications for this product

Product Wall

The immunogen of this antibody is from aa362 - 374 so this antibody should bind to isoform 3 (FGF R2 IIIb) as well. The immunogen is shared between different isoform so please note this product cannot be used to distinguish the isoforms of FGFR2.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount 30 µg
Specification Melanoma cancer
Treatment 5gy Irradiation
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 Jan 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Skin section)
Specification Skin section
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jul 03 2012

Thank you for contacting us.


The only region which differentiated FGFR2b (SwissProt reference P21802-3) and FGFR2c (SwissProt reference P21802) is between residues 313 and 354 of human FGFR2, as illustrated in the attachement of this em...

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Thank you for contacting us and your interest in our products.

The immunogen used to raise anti-FGFR2 antibody (ab10648) is of the sequence APGREKEITASPD which corresponds to amino acid residues 362-374 of human FGFR2. Based on this immunogen...

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Thanks for your email.
Earlier today I sent you and email containing instructions on returning an antibody to Abcam. Below, please find those same instructions:
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Included in this email are the instructions for returning your i...

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Thank you for contacting Abcam. Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
- Return in the original Abcam box with the cooler bo...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements on the following order numbers:
You will receive ab7192 to replace ab824
....

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Thanks for your reply.
I'll just need to locate your order information for ab10648 and ab824 on our system so I can process the replacement order.
If you could provide me with:
1) The PO number or Abcam order reference number associated ...

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I would be happy to send along replacement antibodies to replace those not working as expected.
Based on your last reply, it seemed that ab10648 and ab824 were not detecting protein in the expected subcellular region. The report in your email also...

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