概述

  • 产品名称Anti-FGF9抗体
    参阅全部 FGF9 一抗
  • 描述
    兔多克隆抗体to FGF9
  • 经测试应用适用于: IHC-P, WB, ELISA, Neutralisingmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Highly pure (>98%) recombinant mFGF-9 (mouse Fibroblast Growth Factor-9).

性能

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应用

Our Abpromise guarantee covers the use of ab9743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution. PubMed: 17192470
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9 (using 100µl/well antibody solution).
Neutralising Use a concentration of 0.025 - 0.06 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mFGF-9 (1.5 ng/ml).

靶标

  • 功能May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
  • 组织特异性Glial cells.
  • 疾病相关Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 翻译后修饰Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
    N-glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FGF 9 antibody
    • FGF-9 antibody
    • FGF9 antibody
    • FGF9_HUMAN antibody
    • Fibroblast growth factor 9 antibody
    • GAF antibody
    • Glia Activating Factor antibody
    • Glia-activating factor antibody
    • HBFG 9 antibody
    • HBFG9 antibody
    • HBGF-9 antibody
    • Heparin-binding growth factor 9 antibody
    • MGC119914 antibody
    • MGC119915 antibody
    • SYNS3 antibody
    see all

Anti-FGF9 antibody 图像

  • All lanes : Anti-FGF9 antibody (ab9743) at 1/500 dilution

    Lane 1 : Recombinant FGF9
    Lane 2 : Rat cerebral cortex membrane whole tissue lysate. at 15 µg

    Secondary
    Mouse anti-rabbit IgG monoclonal at 1/1000 dilution
    Developed using the ECL technique

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab9743 staining FGF9 in mouse lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

Anti-FGF9 antibody (ab9743)参考文献

This product has been referenced in:
  • Arnaud-Dabernat S  et al. FGFR3 contributes to intestinal crypt cell growth arrest. J Cell Physiol 216:261-8 (2008). Read more (PubMed: 18286540) »
  • Arnaud-Dabernat S  et al. FGFR3 is a negative regulator of the expansion of pancreatic epithelial cells. Diabetes 56:96-106 (2007). IHC-P ; Mouse . Read more (PubMed: 17192470) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lymph node)
Specification Lymph node
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Verified customer

提交于 Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (brain)
Loading amount 15 µg
Specification brain
Gel Running Conditions Reduced Denaturing (gel 15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 16°C
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提交于 Apr 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"