概述

  • 产品名称Anti-FGF8抗体
    参阅全部 FGF8 一抗
  • 描述
    兔多克隆抗体to FGF8
  • 特异性No cross reactivity with other proteins.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide corresponding to a sequence at the C-terminal of Human FGF8.

性能

应用

Our Abpromise guarantee covers the use of ab81384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.
  • 疾病相关Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
    Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 发展阶段In adults expression is restricted to the gonads.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIGF antibody
    • Androgen induced growth factor antibody
    • Androgen-induced growth factor antibody
    • FGF 8 antibody
    • FGF-8 antibody
    • FGF8 antibody
    • FGF8_HUMAN antibody
    • Fibroblast growth factor 8 (androgen induced) antibody
    • Fibroblast growth factor 8 antibody
    • Fibroblast growth factor 8 precursor antibody
    • HBGF 8 antibody
    • HBGF-8 antibody
    • HBGF8 antibody
    • Heparin-binding growth factor 8 antibody
    • HH6 antibody
    • KAL6 antibody
    see all

Anti-FGF8 antibody 图像

  • ab81384 staining FGF8 in Human Ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 37°C; antigen retrieval was by microwave heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 μg/mL) for 2 hours at 37°C. A Biotin-conjugated secondary antibody was used.

  • ab81384 at 1µg/ml staining FGF8 in Rat ovary tissue sections by Immunohistochemistry(Formalin/ PFA-fixed paraffin-embedded sections). The tissue underwent heat mediated antigen retrieval. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

  • All lanes : Anti-FGF8 antibody (ab81384)

    Lane 1 : Rat Ovary Tissue Lysate
    Lane 2 : Rat Ovary Tissue Lysate


    Predicted band size : 27 kDa

Anti-FGF8 antibody (ab81384)参考文献

This product has been referenced in:
  • Hetzl AC  et al. Fibroblast growth factor, estrogen, and prolactin receptor features in different grades of prostatic adenocarcinoma in elderly men. Microsc Res Tech 76:321-30 (2013). Read more (PubMed: 23362007) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step 1% BSA and 5%NGS as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Tissue sections (Embryonic brain tissue)
Specification Embryonic brain tissue
Permeabilization Yes - Heated Citrate Buffer pH 6.0
Fixative Formaldehyde
Username

Michael Rudy

Verified customer

提交于 May 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"