概述

  • 产品名称Anti-FGF5抗体
    参阅全部 FGF5 一抗
  • 描述
    兔多克隆抗体to FGF5
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rabbit, Cow, Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FGF5.

  • 阳性对照
    • This anitbody gave a positive signal in the following tissues lysates: E14 Mouse Embryo Brain; E16 Ms Embryo Brain; E18 Ms Embryo Brain This antibody gave a positive result when used in the following formaldehyde fixed cell lines: NIH-3T3.

性能

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应用

Our Abpromise guarantee covers the use of ab88118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-FGF5 antibody 图像

  • ICC/IF image of ab88118 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab88118 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-FGF5 antibody (ab88118) at 1 µg/ml

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E16 Ms Embryo Brain Tissue Lysate
    Lane 3 : E18 Ms Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-FGF5 antibody (ab88118)参考文献

This product has been referenced in:
  • Ding BS  et al. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci 128:2983-8 (2015). IHC ; Human . Read more (PubMed: 26116571) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"