Synthetic peptide derived from internal sequence of human FGF22.
- Human ovary tissue. Extracts from Jurkat cells and HT-29 cells. MCF-7 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 19 kDa.
||1/50 - 1/100.
||1/500 - 1/1000.
May be involved in hair development.
Belongs to the heparin-binding growth factors family.
Information by UniProt
- FGF 22 antibody
- FGF-22 antibody
- FGF22 antibody
Western blot - FGF22 antibody (ab74860)
All lanes :
Anti-FGF22 antibody (ab74860) at 1/500 dilutionLane 1 :
Extracts from Jurkat cellsLane 2 :
Extracts from HT-29 cellsLane 3 :
Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.Predicted band size:
19 kDaObserved band size:
21 kDa (why is the actual band size different from the predicted?
)Additional bands at:
55 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF22 antibody (ab74860)
Immunohistochemistry analysis of FGF22 in paraffin-embedded
human ovary tissue using ab74860, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunocytochemistry/ Immunofluorescence - FGF22 antibody (ab74860)
Immunofluorescence analysis of FGF22 in MCF-7 cells using ab74860, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
has not yet been referenced specifically in any publications.
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