概述

  • 产品名称Anti-FGF22抗体
    参阅全部 FGF22 一抗
  • 描述
    兔多克隆抗体to FGF22
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from internal sequence of human FGF22.

  • 阳性对照
    • Human ovary tissue. Extracts from Jurkat cells and HT-29 cells. MCF-7 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/60000.
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

Anti-FGF22 antibody 图像

  • All lanes : Anti-FGF22 antibody (ab74860) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from HT-29 cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of FGF22 in paraffin-embedded human ovary tissue using ab74860, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescence analysis of FGF22 in MCF-7 cells using ab74860, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-FGF22 antibody (ab74860)参考文献

ab74860 has not yet been referenced specifically in any publications.

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