概述

  • 产品名称Anti-FGF21抗体
    参阅全部 FGF21 一抗
  • 描述
    兔多克隆抗体to FGF21
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human FGF21.

    (Peptide available as ab66563.)

  • 阳性对照
    • This antibody gave a positive signal on Full length FGF-21 Recombinant Protein (Human)

性能

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应用

Our Abpromise guarantee covers the use of ab66564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 5 µg/ml.
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    Anti-FGF21 antibody 图像

    • Anti-FGF21 antibody (ab66564) at 1 µg/ml + Recombinant human FGF21 protein (ab54141) at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 22 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
    • IHC image of FGF21 staining in Human Liver Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66564, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-FGF21 antibody (ab66564)参考文献

    This product has been referenced in:
    • Yang C  et al. Activation of Liver FGF21 in hepatocarcinogenesis and during hepatic stress. BMC Gastroenterol 13:67 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23590285) »

    See 1 Publication for this product

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