Ab64857 is not guaranteed for WB with endogenous protein since Ab64857 has been batch tested in WB using recombinant full length FGF21 protein only and not all batches detect endogenous FGF21 protein in WB.
For endogenous FGR21, we recommend RabMAb ab171941 (Anti-FGF21 antibody [EPR8314(2)])
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Anti-FGF21 antibody (ab64857) at 1 µg/ml + Recombinant human FGF21 protein (ab54141) at 0.1 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa Observed band size : 20 kDa (why is the actual band size different from the predicted?) Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.ab64857 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of 19.5 kDa.
Dekker Nitert M et al. Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia. Reprod Biol Endocrinol13:14 (2015).
Read more (PubMed: 25890271) »
Omar BA et al. Fibroblast growth factor 21 (FGF21) and glucagon-like peptide 1 contribute to diabetes resistance in glucagon receptor-deficient mice. Diabetes63:101-10 (2014).
Read more (PubMed: 24062250) »