Customer reviews and FAQs

概述

  • 产品名称Anti-FGF2抗体[MC-GF1]
    参阅全部 FGF2 一抗
  • 描述
    小鼠单克隆抗体[MC-GF1] to FGF2
  • 特异性GF1 recognises basic fibroblast growth factor (bFGF).
  • 经测试应用适用于: ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Pig
  • 免疫原

    Recombinant full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-Fr 1/250. PubMed: 17327226Fix with acetone. Not suitable for detecting bFGF bound to cell surface receptors.

靶标

  • 功能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • 组织特异性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 翻译后修饰Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 细胞定位Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    Anti-FGF2 antibody [MC-GF1] 图像

    • Immunohistochemistry (Frozen sections) - Anti-FGF basic antibody [MC-GF1] (ab181)
      Immunohistochemistry (Frozen sections) - Anti-FGF basic antibody [MC-GF1] (ab181)Image from Morss AS et al., J Biol Chem. 2007 May 11;282(19):14635-44. Epub 2007 Feb 27. Fig 7.; doi: 10.1074/jbc.M608565200; May 11, 2007 The Journal of Biological Chemistry, 282, 14635-14644.
      ab181 staining FGF basic in normal (upper) and diabetic (lower) porcine aortic tissue by Immunohistochemistry (Frozen sections).

      Tissue was fixed with acetone and air dried for 24 hours. Sections were blocked for endogenous peroxidase activity and incubated with primary antibody at 1/250 dilution. Staining was detected using DAB and counterstained with hematoxylin.

    Anti-FGF2 antibody [MC-GF1] (ab181)参考文献

    This product has been referenced in:
    • He W  et al. Blockade of Wnt/ß-catenin signaling by paricalcitol ameliorates proteinuria and kidney injury. J Am Soc Nephrol 22:90-103 (2011). WB ; Mouse . Read more (PubMed: 21030600) »
    • Hsu MY  et al. Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation. Lab Invest 88:842-55 (2008). Read more (PubMed: 18560367) »

    See all 4 Publications for this product

    发表研究结果有使用 ab181？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab181 的 abreview 提交 ab181 的问题

    11 Abreviews or Q&A

    10
    Votes: Highest First

    Flow Cytometry abreview for Anti-FGF2 antibody [MC-GF1]

     Good
    Abreviews
    Application Flow Cytometry
    Sample Mouse Cell (Primary hepatocytes)
    Permeabilization Yes - Fluidigm Fix and Perm Buffer
    Gating Strategy Positive cells for APC
    Specification Primary hepatocytes
    Preparation Cell harvesting/tissue preparation method: Liver dissection, followed by mashing the liver to obtain hepatocytes
    Sample buffer: PBS + 10% Bovine Serum
    Fixation Formaaldehyde
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    Abcam user community

    Verified customer

    提交于 May 28 2016

    I checked your website but was not able to find the information I needed about Your anti-bFGF antibody AB181. Is it possible to give me more information about the pI and the sequence of this antibody?
    Thank you in advance!

    Thank you for your inquiry.
    The immunogen used to raise the anti-FGF basic antibody ab181 is the recombinant full length protein(human).
    Unfortunately, the epitope of this clone has not been mapped and the pI of our antibodiesare not determined...

    Read More

    Hello, I do not have the order confirmation number but this was placed under PO# xxxx

    Thank you for your reply.

    I have searched this for this PO and the product's order history an cannot find any order using this PO or to Pfizer placed within the last two years. Was this product purchased from an outside distributor?

    I...

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    Thanks for the information. That is really unfortunate, since that is the reason we purchased this particular antibody.

    Thank you for your reply.

    We are very sorry about this. We will happily replace or refund this product for you.

    I look forward to your reply on how you like to proceed. Please do not hesitate to contact us if you need any more advic...

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    Customer kindly contacted us to inquire about the reference data used for the claim that this product is competitively displaced by heparin.

    Thank you for your patience as I contacted our lab regarding your inquiry.

    I had looked through data regarding this product and found no information to support the statement on the datasheet. These finding were passed onto the production lab ...

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    Customer kindly contacted us to inquire about the reference data used for the claim that this product is is competitively displaced by heparin.

    Thank you very much for contacting us.

    I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

    I am very sorr...

    Read More

    Would like to test in IHC-P with pig sections.

    DISCOUNT CODE: ***
    Expiration date: November 6, 2012

    This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forab181 and include this code in the “Additional Commen...

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    Dear,Some time ago I ordered different monoclonal anti-bFGF antibodies: Ab 181, Ab 17505, Ab 18629, Ab 18631, Ab 18634 and Ab 10419. I was wondering if you have any information about their binding to the bFGF, do they bind to the same epitopes or can I...

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    I have received further information from lab. Unfortunately lab has confirmed that because they do not have any tested data of these antibodies being used as pairs in sELISA and also due to non availability of epitope sequence they are unable to confir...

    Read More

    Dear,Some time ago I ordered different monoclonal anti-bFGF antibodies: Ab 181, Ab 17505, Ab 18629, Ab 18631, Ab 18634 and Ab 10419. I was wondering if you have any information about their binding to the bFGF, do they bind to the same epitopes or can I...

    Read More

    Thank you for your email.

    We unfortunately have not tested any of these antibodies in sandwich ELISA so we will not be able to confirm their use. Technically, sELISA application needs two antibodies (sELISA pair) which should be raised agains...

    Read More

    we need to know the concentration of use of ab181. Thanks and regards

    Thank you for your enquiry. For use in IHC a suggested working dilution would be 10 ug/ml or 1/100. We would, however, recommend that the end user titrates the antibody around this point to optimise it for their own use.

    1-10 of 11 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"