概述

  • 产品名称
    Anti-FGF2抗体[FB-8]
    参阅全部 FGF2 一抗
  • 描述
    小鼠单克隆抗体[FB-8] to FGF2
  • 特异性
    Does not cross-react with human fibroblast growth factor - acidic (aFGF).
  • 经测试应用
    适用于: RIA, ELISA, Dot blot, Neutralising, WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Recombinant full length protein (Human).

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab16828 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab10419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21995307

靶标

  • 功能
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • 组织特异性
    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 序列相似性
    Belongs to the heparin-binding growth factors family.
  • 翻译后修饰
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 细胞定位
    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

文献

This product has been referenced in:
  • De Benedictis L  et al. Rosiglitazone reverses increased duodenal inhibitory response in spontaneously hypertensive rats. Neurogastroenterol Motil 24:e56-66 (2012). IHC-FoFr ; Rat . Read more (PubMed: 21995307) »
  • Wang L  et al. A novel monoclonal antibody to fibroblast growth factor 2 effectively inhibits growth of hepatocellular carcinoma xenografts. Mol Cancer Ther 11:864-72 (2012). ELISA ; Human . Read more (PubMed: 22351746) »

See all 2 Publications for this product

客户评价及客户问答

I have received further information from lab. Unfortunately lab has confirmed that because they do not have any tested data of these antibodies being used as pairs in sELISA and also due to non availability of epitope sequence they are unable to confir...

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Thank you for your email.

We unfortunately have not tested any of these antibodies in sandwich ELISA so we will not be able to confirm their use. Technically, sELISA application needs two antibodies (sELISA pair) which should be raised agains...

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Gracias por contactarnos de nuevo.

¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y gu...

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Thank you for contacting us with your question.

We have 3 different lots in stock and as the concentration varies between these lots, I have created individual documents for each lot. If you require a document for a different lot, please let ...

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Thank you for contacting us. We only provide certificate of compliance for our products. Please find attached the requested certificates. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advic...

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Thank you for your enquiry. Ab10419 is only available unconjugated. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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