Anti-FGF2抗体(ab10420)

概述

  • 产品名称Anti-FGF2抗体
    参阅全部 FGF2 一抗
  • 描述
    兔多克隆抗体to FGF2
  • 特异性Does not cross react with recombinant mouse and human IL6, recombinant human IL1 alpha and IL1 beta, recombinant human TNF alpha, recombinant human IL3, and recombinant human FGF acidic.
  • 经测试应用适用于: Dot blot, ELISA, Neutralising, WB, RIAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab10420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot 1/10000.
ELISA Use at an assay dependent dilution.
Neutralising Use a concentration of 5 µg/ml. 5 µg/ml neutralizes 50% of the biological activity of 1 unit of bFGF using the mouse fibroblast cell line 3T3.
WB Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.
RIA Use at an assay dependent dilution.

靶标

  • 功能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • 组织特异性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 翻译后修饰Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 细胞定位Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic fibroblast growth factor antibody
    • Basic fibroblast growth factor bFGF antibody
    • BFGF antibody
    • FGF 2 antibody
    • FGF B antibody
    • FGF-2 antibody
    • Fgf2 antibody
    • FGF2 basic antibody
    • FGF2_HUMAN antibody
    • FGFB antibody
    • Fibroblast growth factor 2 (basic) antibody
    • Fibroblast growth factor 2 antibody
    • Fibroblast growth factor, basic antibody
    • HBGF 2 antibody
    • HBGF-2 antibody
    • HBGF2 antibody
    • HBGH 2 antibody
    • HBGH2 antibody
    • Heparin binding growth factor 2 precursor antibody
    • Heparin-binding growth factor 2 antibody
    • Prostatropin antibody
    see all

Anti-FGF2 antibody (ab10420)参考文献

ab10420 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.This antibody is usually diluted in tissue culture medium containing 10% serum, or in buffered saline containing 1% BSA depending upon your application. We don't have any particular information on the stability of this antib...

Read More

Thank you for your phone call. I have found out that the tube you received was mislabeled as monoclonal instead of polyclonal. I have confirmed that ab10420 is indeed a polyclonal antibody as stated on our online datasheet. I apologize for this erro...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"