Anti-FGF19抗体(ab85042)

概述

  • 产品名称Anti-FGF19抗体
    参阅全部 FGF19 一抗
  • 描述
    兔多克隆抗体to FGF19
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FGF19.

    (Peptide available as ab93789.)

  • 阳性对照
    • This antibody gave a positive signal in Human liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab85042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

靶标

  • 功能Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB.
  • 组织特异性Expressed in fetal brain, cartilage, retina, and adult gall bladder.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FGF 19 antibody
    • FGF-19 antibody
    • FGF15 antibody
    • FGF19 antibody
    • FGF19_HUMAN antibody
    • Fibroblast growth factor 15 antibody
    • Fibroblast growth factor 19 antibody
    see all

Anti-FGF19 antibody 图像

  • Anti-FGF19 antibody (ab85042) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab85042 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85042, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FGF19 antibody (ab85042)参考文献

ab85042 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. No, the discount code does not need to be mentioned when placing the first order. Once your Abreview is submitted, the discount code can be redeemed on a future order for a free primary antibody of your choice.

Abreviews
Application Western blot
Sample Pig Tissue lysate - whole (Ileum)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 60 µg
Specification Ileum
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Jul 25 2016

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Pig Cell lysate - whole cell (Porcine FGF19 protein overexpressed in HEK293 cell)
Specification Porcine FGF19 protein overexpressed in HEK293 cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Mar 24 2015

DISCOUNT CODE: ***
Expiration date: December 7th, 2012

I am very pleased to hear you would like to accept our offer and test ab85042 in chicken. This code will give you: 1 free primary antibody before the expiration date. To redeem this ...

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Thank you for contacting us. Of the FGF19 antibodies that are currently in our catalog, I would recommend ab85042, which is 87% homologous with the chicken (NP_990005.1).

To our knowledge, ab85042 has not been tested in chicken. Therefore, I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"