Anti-FGF18抗体(ab63037)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FGF18
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF18.

    (Peptide available as ab91611.)

  • 阳性对照
    • This antibody gave a positive signal in human skin and small intestine tissue lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).

靶标

  • 功能
    Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation.
  • 序列相似性
    Belongs to the heparin-binding growth factors family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FGF 18 antibody
    • FGF-18 antibody
    • Fgf18 antibody
    • FGF18_HUMAN antibody
    • FGFI antibody
    • Fibroblast growth factor 18 antibody
    • zFGF5 antibody
    see all

图片

  • All lanes : Anti-FGF18 antibody (ab63037) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes


    The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to human Fibroblast growth factor 18 precursor (FGF18). Furthermore FGF18 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

实验方案

文献

ab63037 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you very much for your interest in Abcam FGF18 antibodies.

The immunogen of http://www.abcam.com/ab63037 has 82% homology with the Chicken protein. However, immunogens of both http://www.abcam.com/ab86571 and http://www.abcam.com/ab13390...

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Thank you for your enquiry regarding ab63037. The immunogen used to raise this antibody was a synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human FGF18. Unfortunately, we are not able to release the exact im...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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