概述

  • 产品名称
  • 描述
    兔多克隆抗体to FGF10
  • 经测试应用
    适用于: WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Non human primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human FGF10.

    (Peptide available as ab90831.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Lung Tissue; A549 Whole Cell

性能

应用

Our Abpromise guarantee covers the use of ab71794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 23 kDa).
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to FGF10 (ab90207). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab90207 as Capture.

靶标

  • 功能
    Could be a growth factor active in the process of wound healing. Acts as a mitogen in the lung. May act in a manner similar to FGF-7.
  • 疾病相关
    Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG) [MIM:180920]. ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.
    Defects in FGF10 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
  • 序列相似性
    Belongs to the heparin-binding growth factors family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BB213776 antibody
    • fd11d03 antibody
    • FGF 10 antibody
    • FGF-10 antibody
    • FGF10 antibody
    • FGF10_HUMAN antibody
    • Fibroblast growth factor 10 antibody
    • Keratinocyte growth factor 2 antibody
    • KGF 2 antibody
    • Produced by fibroblasts of urinary bladder lamina propria antibody
    • wu:fd11d03 antibody
    • zgc:109774 antibody
    see all

Anti-FGF10 antibody 图像

  • Standard Curve for FGF10 (Analyte: ab73195) dilution range 1pg/ml to 1ug/ml using Capture Antibody Goat polyclonal to FGF10 (ab90207) at 5ug/ml and Detector Antibody Rabbit polyclonal to FGF10 (ab71794) at 0.5ug/ml
  • All lanes : Anti-FGF10 antibody (ab71794) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 170 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

实验方案

Anti-FGF10 antibody (ab71794)参考文献

This product has been referenced in:
  • Chen X  et al. Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis. Mol Med Rep 14:2929-36 (2016). WB ; Mouse . Read more (PubMed: 27510750) »
  • MacKenzie B  et al. Attenuating endogenous Fgfr2b ligands during bleomycin-induced lung fibrosis does not compromise murine lung repair. Am J Physiol Lung Cell Mol Physiol 308:L1014-24 (2015). Read more (PubMed: 25820524) »

See all 2 Publications for this product

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