概述

性能

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应用

Our Abpromise guarantee covers the use of ab9588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hFGF1 (30.0 ng/ml), a concentration of 0.46 - 0.9 µg/ml of this antibody is required.
IHC-Fr 1/200.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • 序列相似性Belongs to the heparin-binding growth factors family.
  • 细胞定位Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acidic fibroblast growth factor antibody
    • aFGF antibody
    • Beta endothelial cell growth factor antibody
    • Beta-endothelial cell growth factor antibody
    • ECGF antibody
    • ECGF beta antibody
    • ECGF-beta antibody
    • ECGFA antibody
    • ECGFB antibody
    • Endothelial Cell Growth Factor alpha antibody
    • Endothelial Cell Growth Factor beta antibody
    • FGF 1 antibody
    • FGF alpha antibody
    • Fgf1 antibody
    • FGF1_HUMAN antibody
    • FGFA antibody
    • Fibroblast Growth Factor 1 Acidic antibody
    • Fibroblast growth factor 1 antibody
    • GLIO703 antibody
    • HBGF 1 antibody
    • HBGF-1 antibody
    • HBGF1 antibody
    • Heparin binding growth factor 1 antibody
    • Heparin binding growth factor 1 precursor antibody
    • Heparin-binding growth factor 1 antibody
    see all

Anti-FGF1 antibody 图像

  • ICC/IF image of ab9588 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab9588 staining FGF1 in rat dorsal root ganglia tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and permeabilized using 0.25% Triton X-100. Samples were then blocked 0.25% BSA for 1 hour at 25°C, then incubated with ab9588 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to rhodamine, 1/500 dilution.

    See Abreview

Anti-FGF1 antibody (ab9588)参考文献

This product has been referenced in:
  • Li M  et al. FGF1 Mediates Overnutrition-Induced Compensatory ß-Cell Differentiation. Diabetes 65:96-109 (2016). IF ; Zebrafish . Read more (PubMed: 26420862) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (DRG)
Specification DRG
Fixative Paraformaldehyde
Permeabilization Yes - 0,25% Trt-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Username

Mrs. Duygu Daglikoca

Verified customer

提交于 Jun 06 2011

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Loading amount 20 µg
Specification Heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 02 2008

This antibody has never been tested in IHC on formalin fixed paraffin embedded sections, therfore we can't guarantee results.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"