Anti-FGF 23抗体(ab123502)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to FGF 23
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide with sequence C-

    ENGYDVYLSQKHH

    , corresponding to internal sequence amino acids 121-133 of Mouse FGF 23 (NP_073148.1)

  • 阳性对照
    • Mouse Brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab123502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 27, 38 kDa (predicted molecular weight: 28 kDa). When using mouse brain lysate:

靶标

  • 功能
    Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
  • 组织特异性
    Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).
  • 疾病相关
    Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
    Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues.
  • 序列相似性
    Belongs to the heparin-binding growth factors family.
  • 翻译后修饰
    Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
    O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23.
  • 细胞定位
    Secreted. Secretion is dependent on O-glycosylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADHR antibody
    • FGF-23 antibody
    • Fgf23 antibody
    • FGF23_HUMAN antibody
    • FGFN antibody
    • Fibroblast growth factor 23 antibody
    • Fibroblast growth factor 23 C-terminal peptide antibody
    • Fibroblast growth factor 23 precursor antibody
    • HPDR2 antibody
    • HYPF antibody
    • Phosphatonin antibody
    • PHPTC antibody
    • Tumor derived hypophosphatemia inducing factor antibody
    • Tumor-derived hypophosphatemia-inducing factor antibody
    see all

图片

  • Anti-FGF 23 antibody (ab123502) at 0.5 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 28 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab123502 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample
Mouse Tissue sections (Femoral bone)
Specification
Femoral bone
Permeabilization
Yes - Tween 20 0.1% in TBS
Fixative
Formaldehyde
Username

Mr. Vasily Gnyubkin

Verified customer

提交于 Jan 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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