FFN102 (Mini 102) (ab120866)

概述

  • 产品名称FFN102 (Mini 102)
  • 描述
    Novel pH responsive FFN. Measures localization and activity of dopaminergic presynaptic terminals.
  • 生物学描述Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
    Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
    Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags.
  • 纯度> 98%

性能

    FFN102 (Mini 102) 图像

    • All images provided by Thorsten Lau

      Figure 1: Two neuronal cells stained with 50 μM FFN102 on differentiation day 10. Shown is a sum projection of a confocal z-stack. Accumulation of FFN102 can be observed along the neurites (arrows) and the cell soma (S).

    • Figure 2a: Images in the first row show a group of neuronal cells stained with 50 μM FFN102 (sum projection of a confocal stack). FFN102 localizes to structures on the cell soma (S) as well as neurites (arrows). Z indicates the area zoomed in for an additional z-stack. 

    • Figure 2b: Zoomed in area of “Z” from figure 2b. The arrow indicates a globular structure on a neurite. S indicates FFN102 positive structures on the cell soma.
    • Figure 3: FFN102 dye uptake inhibition on addition of VMAT2 inhibitor RO4-1284

    FFN102 (Mini 102) (ab120866)参考文献

    This product has been referenced in:
    • Rodriguez PC  et al. Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain. Proc Natl Acad Sci U S A 110:870-5 (2013). Read more (PubMed: 23277566) »

    See 0 Publications for this product

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