• 产品名称Anti-FFAR3抗体
    参阅全部 FFAR3 一抗
  • 描述
    兔多克隆抗体to FFAR3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 301-331 of Human FFAR3.

  • 阳性对照
    • Jurkat cell line lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell line: T24/83.



Our Abpromise guarantee covers the use of ab103718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 5 µg/ml.


  • 功能Receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation. Coupled exclusively to the pertussis toxin-sensitive G(i/o)-alpha protein. The rank order of potency for agonists of this receptor is propionate = pentanoate = butyrate > acetate > formate.
  • 组织特异性Highest level in adipose tissue, and lower expression across all tissues tested.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FFA3R antibody
    • Ffar3 antibody
    • FFAR3_HUMAN antibody
    • Free fatty acid receptor 3 antibody
    • G protein coupled receptor 41 antibody
    • G-protein coupled receptor 41 antibody
    • gpcr41 antibody
    • GPR41 antibody
    • gpr42 antibody
    see all

Anti-FFAR3 antibody 图像

  • ICC/IF image of ab103718 stained T24/83 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103718 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FFAR3 antibody (ab103718) at 1/100 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 39 kDa

Anti-FFAR3 antibody (ab103718)参考文献

ab103718 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Sample Rabbit Cell (GPR41 transfacted CHO-K1 cell)
Specification GPR41 transfacted CHO-K1 cell
Permeabilization Yes - 0.1% tween 20
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Apr 01 2015

Gracias por contactarnos de nuevo.
El péptido usado para generar el anticuerpo es el producto ab128002, podéis echarle un vistazo a su datasheet:
Si necesitas cualquier otra información, p...

Read More

Thank you for contacting us.

I apologize for the time that it has taken to get this onto our catalog. We do now offer the blocking peptide ab103718 anti-FFAR3 (C-term). This product number is ab128002 and can be located at the following li...

Read More