概述

  • 产品名称
  • 描述
    山羊多克隆抗体to Fez1
  • 特异性
    ab53562 is expected to recognize isoform 1 (NP_005094.1) only.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    C-QNKQKEQRELMKK

    , corresponding to internal sequence amino acids 278-290 of Human Fez1

  • 阳性对照
    • Human Brain (frontal Cortex) lysate.

性能

应用

Our Abpromise guarantee covers the use of ab53562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能
    May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules.
  • 组织特异性
    Mainly expressed in brain.
  • 序列相似性
    Belongs to the zygin family.
  • 翻译后修饰
    Phosphorylated by protein kinase C zeta; which enhances interaction with UBE4B and polyubiquitination.
    Polyubiquitinated in a UBE4B-dependent manner; which does not lead to proteasomal degradation and may be important for neurogenic activity. Polyubiquitin linkage seems to be mainly through Lys-26.
  • 细胞定位
    Cytoplasm > cytoskeleton > centrosome. Cell membrane. Co-localizes with both, alpha- and gamma-tubulin. Translocated from the plasma membrane to the cytoplasm by activation of the PKC zeta.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Fasciculation and elongation protein zeta 1 antibody
    • Fasciculation and elongation protein zeta-1 antibody
    • Fez 1 antibody
    • Fez antibody
    • FEZ1 antibody
    • FEZ1_HUMAN antibody
    • Fezl antibody
    • Zygin 1 antibody
    • Zygin I antibody
    • Zygin-1 antibody
    • Zygin1 antibody
    • ZyginI antibody
    see all

Anti-Fez1 antibody 图像

  • Anti-Fez1 antibody (ab53562) at 0.03 µg/ml + Human Brain (frontal Cortex) lysate (in RIPA buffer) at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

实验方案

Anti-Fez1 antibody (ab53562)参考文献

This product has been referenced in:
  • McKnight NC  et al. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J : (2012). WB ; Human . Read more (PubMed: 22354037) »

See 1 Publication for this product

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