纯化说明Ab34505 was purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: 1/40,000 - 1/200,000.
WB: 1/10000 - 1/20000. Detects a band of approximately 58 kDa.
Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
组织特异性Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
序列相似性Belongs to the fetuin family. Contains 2 cystatin fetuin-A-type domains.
翻译后修饰Phosphorylation sites are present in the extracelllular medium. O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).