Anti-Fetuin A抗体[MM0273-6M23] (ab89227)
Key features and details
- Mouse monoclonal [MM0273-6M23] to Fetuin A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Fetuin A抗体[MM0273-6M23]
参阅全部 Fetuin A 一抗 -
描述
小鼠单克隆抗体[MM0273-6M23] to Fetuin A -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Human recombinant Fetuin A
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阳性对照
- WB: Human placenta tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography. -
克隆
单克隆 -
克隆编号
MM0273-6M23 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89227于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 39 kDa.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 39 kDa. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. -
组织特异性
Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. -
序列相似性
Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. -
翻译后修饰
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 197 Human
- Omim: 138680 Human
- SwissProt: P02765 Human
- Unigene: 324746 Human
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别名
- 59 kDa bone sialic acid containing protein antibody
- A2HS antibody
- Aa2 066 antibody
see all
图片
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Anti-Fetuin A antibody [MM0273-6M23] (ab89227) at 1/500 dilution + Human placenta tissue lysate
Predicted band size: 39 kDa
Observed band size: 39 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling Fetuin A with ab89227. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab89227 被引用在 2 文献中.
- Podracky CJ et al. Laboratory evolution of a sortase enzyme that modifies amyloid-β protein. Nat Chem Biol 17:317-325 (2021). PubMed: 33432237
- Dorr BM et al. Reprogramming the specificity of sortase enzymes. Proc Natl Acad Sci U S A 111:13343-8 (2014). WB ; Human . PubMed: 25187567