Anti-Ferritin Light Chain抗体(ab80585)

概述

  • 产品名称Anti-Ferritin Light Chain抗体
    参阅全部 Ferritin Light Chain 一抗
  • 描述
    山羊多克隆抗体to Ferritin Light Chain
  • 经测试应用适用于: Sandwich ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Cow, Cat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Ferritin Light Chain aa 163-175 (C terminal) (Cysteine residue).
    Sequence:

    C-GEYLFERLTLKHD


    Database link: NP_000137.2

  • 阳性对照
    • WB: Human placenta, human cerebellum, human liver, mouse brain and rat brain tissue lysates. IHC-P: Human brain cortex tissue.

性能

应用

Our Abpromise guarantee covers the use of ab80585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect recombinant FTL
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

靶标

Anti-Ferritin Light Chain antibody 图像

  • All lanes : Anti-Ferritin Light Chain antibody (ab80585) at 0.1 µg/ml

    Lane 1 : Human cerebellum tissue lysate in RIPA buffer
    Lane 2 : Human liver tissue lysate in RIPA buffer
    Lane 3 : Human placenta tissue lysate in RIPA buffer
    Lane 4 : Mouse brain tissue lysate in RIPA buffer
    Lane 5 : Rat brain tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa

    Incubated with the primary antibody for 1 hour.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain cortex tissue labelling Ferritin Light Chain with ab80585 at 3.8µg/ml. Antigen retrieval was performed with citrate buffer pH 6. HRP staining.

Anti-Ferritin Light Chain antibody (ab80585)参考文献

ab80585 has not yet been referenced specifically in any publications.

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