Anti-Ferritin Light Chain抗体(ab80585)

概述

  • 产品名称
    Anti-Ferritin Light Chain抗体
    参阅全部 Ferritin Light Chain 一抗
  • 描述
    山羊多克隆抗体to Ferritin Light Chain
  • 经测试应用
    适用于: Sandwich ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Cow, Cat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Ferritin Light Chain aa 163-175 (C terminal) (Cysteine residue).
    Sequence:

    C-GEYLFERLTLKHD


    Database link: NP_000137.2

  • 阳性对照
    • WB: Human placenta, human cerebellum, human liver, mouse brain and rat brain tissue lysates. IHC-P: Human brain cortex tissue.

性能

应用

Our Abpromise guarantee covers the use of ab80585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect recombinant FTL
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

靶标

  • 功能
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 疾病相关
    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • 序列相似性
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ferritin L chain antibody
    • Ferritin L subunit antibody
    • Ferritin light chain antibody
    • Ferritin light polypeptide antibody
    • ferritin light polypeptide like 3 antibody
    • FRIL_HUMAN antibody
    • FTL antibody
    • LFTD antibody
    • NBIA 3 antibody
    • NBIA3 antibody
    see all

Anti-Ferritin Light Chain antibody 图像

  • All lanes : Anti-Ferritin Light Chain antibody (ab80585) at 0.1 µg/ml

    Lane 1 : Human cerebellum tissue lysate in RIPA buffer
    Lane 2 : Human liver tissue lysate in RIPA buffer
    Lane 3 : Human placenta tissue lysate in RIPA buffer
    Lane 4 : Mouse brain tissue lysate in RIPA buffer
    Lane 5 : Rat brain tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa

    Incubated with the primary antibody for 1 hour.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain cortex tissue labelling Ferritin Light Chain with ab80585 at 3.8µg/ml. Antigen retrieval was performed with citrate buffer pH 6. HRP staining.

Anti-Ferritin Light Chain antibody (ab80585)参考文献

ab80585 has not yet been referenced specifically in any publications.

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