Anti-Ferritin Heavy Chain抗体[EPR3005Y] (ab75972)
- 数据表
- 具体的参考文献 (7)
- 实验方案
概述
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产品名称Anti-Ferritin Heavy Chain抗体[EPR3005Y]
参阅全部 Ferritin Heavy Chain 一抗 -
描述兔单克隆抗体[EPR3005Y] to Ferritin Heavy Chain
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宿主Rabbit
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特异性This antibody recognizes only the Ferritin heavy chain.
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经测试应用适用于: WB, IHC-P, IHC-Frmore details
不适用于: ICC/IF -
种属反应性与反应: Human
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免疫原
Synthetic peptide within Human Ferritin Heavy Chain aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: P02794 -
阳性对照
- WB: Human fetal liver, SH-SY5Y, human brain and HeLa cell lysates. IHC: Human kidney, breast and tonsil tissue.
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常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EPR3005Y
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab75972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 25498860 |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 2495 Human
- Omim: 134770 Human
- SwissProt: P02794 Human
- Unigene: 524910 Human
- Unigene: 645560 Human
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别名
- Apoferritin antibody
- Cell proliferation inducing gene 15 protein antibody
- Cell proliferation-inducing gene 15 protein antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/Ferritin-Heavy-Chain-Primary-antibodies-ab75972-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Unpurified ab75972 showing positive staining in normal breast tissue.
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![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/ab75972-287323-ab75792.jpg)
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)All lanes : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution
Lane 1 : MCF-7 (human breast carcinoma) whole cell lysates prepared from 1% SDS Hot lysis method.
Lane 2 : MCF-7 (human breast carcinoma) whole cell lysates prepared from RIPA lysis method
Lane 3 : HeLa (human cervix adenocarcinoma) whole cell lysates prepared from 1% SDS Hot lysis method
Lane 4 : HeLa (human cervix adenocarcinoma) whole cell lysates prepared from RIPA lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
Ab75972 works better in some lysates prepared from 1% SDS Hot lysis method.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). -
![Immunohistochemistry - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/ab75972-259524-ab75972IHC1.jpg)
Immunohistochemistry - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)ab75972 staining Ferritin Heavy Chain in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/ab75972_1.jpg)
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Lanes 1-3 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution (unpurified)
Lane 4 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution (unpurified)
Lane 5 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution (unpurified)
Lane 1 : Human liver lysate
Lane 2 : SH-SY5Y lysate
Lanes 3-4 : Human brain lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/ab75972-259529-ab75972WB2.jpg)
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution + Human fetal liver at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 21 kDa -
![Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/ab75972-259526-ab75972WB1.jpg)
Western blot - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution + HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/ab75972_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in human kidney tissue, using a HRP/AP polymerized secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/Ferritin-Heavy-Chain-Primary-antibodies-ab75972-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Unpurified ab75972 showing positive staining in normal liver tissue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)](http://www.abcam.cn/ps/products/75/ab75972/Images/Ferritin-Heavy-Chain-Primary-antibodies-ab75972-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972)Unpurified ab75972 showing positive staining in normal tonsil tissue.
文献
This product has been referenced in:
- Mancone C et al. Iron overload down-regulates the expression of the HIV-1 Rev cofactor eIF5A in infected T lymphocytes. Proteome Sci 15:18 (2017). Read more (PubMed: 28785172) »
- Vannucci L et al. In Vivo Targeting of Cutaneous Melanoma Using an Melanoma Stimulating Hormone-Engineered Human Protein Cage with Fluorophore and Magnetic Resonance Imaging Tracers. J Biomed Nanotechnol 11:81-92 (2015). IHC-P ; Human . Read more (PubMed: 26301302) »
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